富硒蛹虫草多糖对鱼藤酮诱导伤害果蝇的保护功效

doi:10.7506/spkx1002-6630-201307056

食品科学 ›› 2013, Vol. 34 ›› Issue (7): 266-269.doi: 10.7506/spkx1002-6630-201307056

富硒蛹虫草多糖对鱼藤酮诱导伤害果蝇的保护功效

孙军德，侯静，杨逸

沈阳农业大学土地与环境学院，辽宁沈阳 110866

收稿日期:2012-03-05 修回日期:2013-03-05 出版日期:2013-04-15 发布日期:2013-03-20
通讯作者: 孙军德 E-mail:sunjunde108@163.com

Protective Effect of Selenium-Enriched Polysaccharides from Cordyceps militaris against Rotenone-Induced Oxidative Damage in Drosophila melanogaster

SUN Jun-de，HOU Jing，YANG Yi

College of Land and Environment, Shenyang Agricultural University, Shenyang 110866, China

Received:2012-03-05 Revised:2013-03-05 Online:2013-04-15 Published:2013-03-20
Contact: SUN Jun-de E-mail:sunjunde108@163.com

摘要/Abstract

摘要： 目的：探讨蛹虫草多糖和富硒蛹虫草多糖在鱼藤酮诱导的果蝇伤害模型中缓解氧化压力和神经毒保护功效。方法：采用鱼藤酮诱导伤害模型，评估饲喂含多糖与不含培养基果蝇的氧化指标和神经伤害。分别测定丙二醛(MDA)、蛋白质羧基化(PC)、谷胱甘肽(GSH和GSSG)、超氧化物歧化酶(SOD)和谷胱甘肽过-S-转移酶(GST)指标；同时测定逆重力爬行、乙酰胆碱酯酶(Ache)和丁酰胆碱酯酶(Bche)活力，对多糖的神经保护作用进行评估。结果：蛹虫草多糖和富硒蛹虫草多糖对降低果蝇体内氧化压力和神经伤害效果显著，相同质量浓度条件下富硒多糖保护效果好于普通蛹虫草多糖，其中1%富硒蛹虫草多糖溶液添加组逆重力爬行能力可回复到对照组的85%，部分氧化压力指标和酶活力也基本恢复至对照组水平。

关键词: 蛹虫草多糖, 硒, 鱼藤酮, 抗氧化, 神经保护

Abstract: Objective: To explore the protective effect of Cordyceps militaris polysaccharide (CPS) and selenium-enriched polysaccharide (SE-CPS) on rotenone-induced oxidative stress and neurotoxicity in Drosophila melanogaster. Methods: Rotenoneinduced fruit fly damage models were divided into basic medium with or without CPS and Se-CPS to evaluate oxidative stress and neurotoxity. Seven antioxidant indicators including malondialdehyde (MDA), protein carboxyl (PC), glutathione content (GSH and GSSG), superoxide dismutase (SOD) and glutathione-S-transferase (GST) were measured. Meanwhile, acetylcholinesterase (Ache,) and butyrylcholinesterase (Bche) activities were also analyzed to evaluate the neuroprotective role of both polysaccharides. Results: Both CPS and Se-CPS could reduce oxidative stress indicators and neurotoxicity. Se-CPS had better protective effect at equal concentrations; 1% Se-CPS could restore the damage to 85% level of control group. Moreover, some oxidative stress indicators and enzyme activity were also restored to the control level.

Key words: Cordyceps polysaccharide, selenium, rotenone, antioxidant, neuroprotective

中图分类号:

TS201.2

孙军德，侯静，杨逸. 富硒蛹虫草多糖对鱼藤酮诱导伤害果蝇的保护功效[J]. 食品科学, 2013, 34(7): 266-269.

SUN Jun-de，HOU Jing，YANG Yi. Protective Effect of Selenium-Enriched Polysaccharides from Cordyceps militaris against Rotenone-Induced Oxidative Damage in Drosophila melanogaster[J]. FOOD SCIENCE, 2013, 34(7): 266-269.

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富硒蛹虫草多糖对鱼藤酮诱导伤害果蝇的保护功效

Protective Effect of Selenium-Enriched Polysaccharides from Cordyceps militaris against Rotenone-Induced Oxidative Damage in Drosophila melanogaster

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