亚硒酸钠促进多形汉逊酵母DL-1合成谷胱甘肽的转录组学分析 王?婷1，刘婵婵2，任?娟1，荆蓉蓉1，钱卫东1,* （1.陕西科技大学食品与生物工程学院，陕西?西安 710021；2.西安医学高等专科学校，陕西?西安 710032） 摘?要：为解析Na2SeO3诱导多形汉逊酵母DL-1（Hansenula polymorpha DL-1，HP-DL-1）生物合成谷胱甘肽（glutathione，GSH）的分子机制。通过DTNB法以及Illumina测序平台对比分析不同浓度Na2SeO3对HP-DL-1合成GSH产量的影响及Na2SeO3诱导下GSH高产菌株与出发菌株转录组差异。结果表明：以60?μmol/L?Na2SeO3诱导HP-DL-1发酵48?h，酵母总GSH产量达（530.22±9.6）mg/L；与对照组相比，Na2SeO3诱导组共有1 254 个显著性差异表达基因（differentially expressed genes，DEGs），其中630?个DEGs表达上调，624?个DEGs表达下调；依据基因本体（Gene Ontology，GO）和KEGG（Kyoto Encyclopedia of Genes and Genomes）富集，这些差异基因主要集中在细胞周期、有丝分裂、氨基酸生物合成、糖酵解、核糖体组分、甲烷、脂肪、核酸、GSH等多条代谢通路。本研究为分子改造构建高产GSH的酵母工程菌株提供一定参考。 多形汉逊酵母；谷胱甘肽；亚硒酸钠；转录组；差异表达基因 Transcriptome Analysis of Hansenula polymorpha DL-1 with Sodium Selenite-Induced Biosynthesis and Accumulation of Glutathione WANG Ting1, LIU Chanchan2, REN Juan1, JING Rongrong1, QIAN Weidong1,* (1. School of Food and Biological Engineering, Shaanxi University of Science & Technology, Xi’an 710021, China; 2. Xi’an Medical College, Xi’an 710032, China) Abstract: To elucidate the mechanism of glutathione (GSH) biosynthesis by Hansenula polymorpha DL-1 (HP-DL-1) exposed to Na2SeO3, we examined the differentially expressed genes (DEGs) in yeast cells treated and not treated with Na2SeO3 using a combination of transcriptomic sequencing and bioinformatic methods. The results showed that 60 μmol/L Na2SeO3 was found to be able to increase the GSH yield of yeast cells up to (530.22 ± 9.6) mg/L after 48 h fermentation. A total of 1 254 DEGs was identified in the Na2SeO3-induced group, of which 630 genes were up-regulated, whereas the remaining 624 genes were down-regulated. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses indicated that these DEGs were involved in the cell cycle, mitosis, amino acid biosynthesis, glycolysis, ribosome components and methane, fat, nucleic acid, GSH metabolism pathways. This study provides adequate information for a better understanding of the physiological mechanism of GSH biosynthesis by H. polymorpha, which will provide theoretical support for subsequent molecular improvement for over-production of GSH by engineered strains. Hansenula polymorpha; glutathione; sodium selenite; transcriptome; differentially expressed genes DOI:10.7506/spkx1002-6630-20200121-252 中图分类号：Q939.97