食品科学 ›› 2006, Vol. 27 ›› Issue (1): 97-100.

• 基础研究 • 上一篇    下一篇

红曲霉M9x的原生质体形成与再生条件的研究

 戴德慧, 蒋家新, 黄光荣   

  1. 浙江科技学院生化系
  • 出版日期:2006-01-15 发布日期:2011-09-05

Study on Formation and Regeneration of the Protoplast from Monascus ruber M9x

 DAI  De-Hui, JIANG  Jia-Xin, HUANG  Guang-Rong   

  1. School of Biological and Chemical Engineering, Zhejiang University of Science and Technology
  • Online:2006-01-15 Published:2011-09-05

摘要: 对红曲霉M9x用混合酶制取原生质体及原生质体再生条件进行了探索。其原生质形成和再生最佳条件为:以豆芽汁培养液为菌丝培养基,采用60h菌龄的菌丝体,以0.6mol/LMgSO4作为渗稳剂,用1.5%混合酶(蜗牛酶:纤维素酶=7:3)在30℃处理2h,原生质体形成数可以达到1.61×107个/ml,再生率为8.49%。

关键词: 红曲霉, 原生质体, 形成, 再生

Abstract: The formation and regeneration of the protoplast from Monascus ruber M9x were studied. The results showed that the soybean sprout juice used to culture mycelium and the mycelium aged 60h used as operated material were easily processed to form protoplast. Under the action of 1.5% mixed enzyme (snail enzyme: cellulose enzyme =7:3) and with 0.6mol/L MgSO4 used as osmotic stabilizer, the protoplasts could reach 1.61×1 07/ml, and the regeneration rate was 8.49%.

Key words: Monascus ruber, protoplast, formation, regeneration