食品科学 ›› 2006, Vol. 27 ›› Issue (11): 171-173.
• 基础研究 • 上一篇 下一篇
袭凌, 李柏林, 欧杰
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XI Ling, LI Bai-Lin, 欧Jie
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摘要: 本文研究了两种不同的连接方法在野油菜黄单胞菌(Xanthomonascampestris)xanA基因与pUCm-T载体连接中的连接效果,并进行了转化实验进行鉴定。实验结果表明,xanA基因与pUCm-T载体的适宜连接条件为16℃连接过夜。连接体系中,xanA基因与pUCm-T载体的体积比为4:1。
关键词: 野油菜黄单胞菌, xanA基因, pUCm-T载体, 连接
Abstract: Two methods for gene xanA to ligate with pUCm-T vector were discussed in this thesis. And the transformations were also done for testing. It showed that the better ligation condition was ligated at 16℃ for overnight. In the ligation system,the volume ratio of gene xanA to pUCm-T vector was 4:1.
Key words:  , Xanthomonas campestris; gene xanA; pUCm-T vector; ligation;
袭凌, 李柏林, 欧杰. 节点文献野油菜黄单胞菌xanA基因与pUCm-T载体的连接[J]. 食品科学, 2006, 27(11): 171-173.
XI Ling, LI Bai-Lin, 欧Jie. The Ligation of Gene xanA in Xanthomonas campestris and pUCm-T Vector[J]. FOOD SCIENCE, 2006, 27(11): 171-173.
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