食品科学 ›› 2006, Vol. 27 ›› Issue (11): 192-194.

• 基础研究 • 上一篇    下一篇

外切蛋白酶作用蛋清蛋白制备高F值寡肽的外切效果研究

 刘静波,  赵法利,  刘瑜,  林松毅   

  1. 吉林大学军需科技学院营养与功能食品研究室
  • 出版日期:2006-11-15 发布日期:2011-11-28

Study on the Effect of Exoproteinase in Preparation of High F Value Oligopetide from Egg White

 LIU  Jing-Bo,   Zhao-Fa-Li,   Liu-Yu,   Lin-Song-Yi   

  1. Laboratory of Nutrition and Functional Food,College of Quartermaster Technology,Jilin University,Changchun 130062,China
  • Online:2006-11-15 Published:2011-11-28

摘要: 以水解度(DH)作为实验的主要衡量指标,通过pH-stat法对pH值、水解温度、水解时间、[E]/[S]等实验条件的考察,确定出最佳水解条件为:pH7.5、水解温度为60℃、[E]/[S]为3.3%、水解时间为5h。高F值化处理过程中,以OD220/OD280为实验的主要衡量指标,通过紫外分光光度法对搅拌温度、搅拌时间、pH值、炭液比进行考察,确定其最优条件为:炭液比1:10、pH值为7、搅拌时间3h、搅拌温度50℃。

关键词: 外切蛋白酶, 高F值

Abstract: Egg white was used as raw material,contrast analysis and orthogonal test to do a system research on the optimal disassembling conditions of enzyme in preparing high F value oligopeptides. The DH was regarded as a basis evaluating index. According to tests results of factors such as pH value,hydrolyzing temperature,time and E/S,meanwhile,an orthogonal design of L9(34) was adopted to draw a conclusion suitable for the hydrolysis of Flavouerzyme as followpH7.5,temperature 60℃,E/S 3.3%,5 hours. The optimal conditions for preparing high F value as followactive carbon to liquid 1:10,temperature 50℃,pH 7 and 3 hours.

Key words: exoproteinase, high F value