食品科学 ›› 2006, Vol. 27 ›› Issue (2): 112-115.

• 基础研究 • 上一篇    下一篇

醪糟发酵液对α—葡萄糖苷酶抑制作用的研究

鲁战会, 杨宁国, 李里特, 李永玉, 许中敏, 陈金显, 朱韶娟   

  1. 中国农业大学食品科学与营养工程学院; 北京维他科技有限公司; 广州普正生物科技有限公司;
  • 出版日期:2006-02-15 发布日期:2011-09-05

Study on Inhibition Effect of Fermented Supernatant of Laozao on α-glucosidase

Lu-Zhan-Hui, YANG  Ning-Guo, LI  Li-Te, LI  Yong-Yu, XU  Zhong-Min, CHEN  Jin-Xian, ZHU  Shao-Juan   

  1. 1.College of Food Science and Nutritional Engineering, China Agricultural University; 2.Beijing Weita Science and Technology Co. Ltd; 3.Guangzhou Procept Biotech Co. Ltd.
  • Online:2006-02-15 Published:2011-09-05

摘要: 通过一系列功能性实验,对醪糟发酵液的抗老年性痴呆、抗氧化、降血压、降血糖等作用进行了初步研究,发现醪糟发酵液抑制血管紧张素转换酶和α-葡萄糖苷酶的作用确切而且明显,具有潜在的降血压和降血糖作用。然后,主要研究了不同产地、不同发酵时间的醪糟发酵液以及它们在不同的稀释度下对α-葡萄糖苷酶的抑制作用,并排除了乙醇的抑制作用。发酵液经灭活、离心处理,用p-NPG(α-葡萄糖苷对硝基苯酚)法测定α-葡萄糖苷酶的活性。结果表明,在原浓度下,不同产地的醪糟发酵液对α-葡萄糖苷酶的抑制效果基本相同;在醪糟发酵的过程中,发酵液对α-葡萄糖苷酶的抑制效果保持稳定,均在60%以上,且在发酵的第60~66h内抑制效果最好。此外,不同稀释度下的醪糟发酵液对α-葡萄糖苷酶的抑制效果不同,随着其浓度的增大,醪糟发酵液的抑制效果明显增强。这说明醪糟发酵液对α-葡萄糖苷酶具有稳定的抑制作用(由于篇幅有限,降血压作用另作介绍)。

关键词: 醪糟发酵液, &alpha, -葡萄糖苷酶, 抑制作用, 降血糖

Abstract: By a series of functionality experiments, the functionality of the fermented supernatant of Laozao (rice distillers’ grain) was studied in this paper, such as to reduce the risks of the Alzheimer disease (AD), antioxidation, hypotension, hypoglycemic. The inhibition effects of α-glucosidase and acetylcholinesterase(AChE) were validated. Then, the inhibition effects of the fermented supernatant of different producing areas and different fermented time and different diluted concentra- tions were studied onα-glucosidase, while the inhibition effect of ethanol was excluded. The fermented supernatant was boiled and centrifuged and the activity of α-glucosidase was assayed by p-NPG(p-nitrophenyl α-D-glucopyranoside). It showed that in primal concentration, the fermented supernatant of the different producing areas had steady inhibition effect on α-glucosidase daring the course of fermentation of Laozao, it also had steady inhibition effect on α-glucosidase and the inhibition rate was higher than 60 percent. Moreover,in the sixtieth to sixty-sixth hour, the inhibition effect is highest. In addition, the different concentrations of fermented supernatant of Laozao had different inhibition effect. When the concentration increased, the inhibition effect increased significantly. This finding suggests that the fermented supernatant of Laozao has steady inhibition effect on α-glucosidase.

Key words: the fermented supernatant of Laozao(rice distillers&rsquo, grain); &alpha, -glucosidase; inhibition; hypoglycemic;