食品科学 ›› 2006, Vol. 27 ›› Issue (2): 166-168.

• 工艺技术 • 上一篇    下一篇

杂色云芝漆酶用于提取人参总皂甙的研究

 郭梅, 白东清, 蒲军, 杜连祥, 路福平   

  1. 天津农学院食品科学系; 天津科技大学生物工程学院工业微生物重点实验室
  • 出版日期:2006-02-15 发布日期:2011-09-05

Extraction Study on Ginsenosides with Laccase

 GUO  Mei, BAI  Dong-Qing, PU  Jun, DU  Lian-Xiang, LU  Fu-Ping   

  1. 1.Department of Food Science, Tianjin Agricultural College, Tianjin 300384, China; 2.Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300222, China;3. Key Laboratory of Ecology and Aquaculture, Department of Fishery Science, Tianjin Agricultural College, Tianjin 300384, China
  • Online:2006-02-15 Published:2011-09-05

摘要: 采用正交试验,对水浸提取条件、漆酶酶解条件进行了研究。结果表明人参总皂甙最佳提取工艺为:先用漆酶酶解,每克人参加入15ml漆酶粗酶液(92U/ml),在30℃、pH3.5的条件下酶解1.5h,然后水浸提取人参总皂甙。经过漆酶酶解处理后人参总皂甙提取率比水浸提取法提高了70.9%。

关键词:  , 漆酶, 酶解, 人参总皂甙, 正交试验

Abstract: The optimum conditions of enzymolysis and extraction process by orthogonal design were studied. The results showed that the optimum conditions of ginsenosides extraction technique were as follows: First, laccase treatment: 1g Panax ginseng was put into 15ml (92U/ml) laccase with temperature 30℃, pH value 3.5 and fermenting time 1.5h. Then, in comparison with water-extraction, the enzyme-based extraction rate of ginsenosides is increased by 70.9%.

Key words:  , laccase; enzymolysis; ginsenosides; orthogonal design;