动物脏体中微量硒的测定

食品科学 ›› 2006, Vol. 27 ›› Issue (4): 202-205.

动物脏体中微量硒的测定

粟智, 车云霞, 高学平, 申泮文

新疆师范大学生命与环境科学学院；南开大学化学学院

出版日期:2006-04-15 发布日期:2011-09-13

Determination of Trace Selenium in Animal Viscera

SU Zhi, CHE Yun-Xia, GAO Xue-Ping, SHEN Pan-Wen

1.Institute of Life and Environment Science, Xinjiang Normal University, Urumqi 830054, China; 2.College of Chemistry, Nankai University, Tianjin 300071, China

Online:2006-04-15 Published:2011-09-13

摘要/Abstract

摘要： 研究了猪、牛、羊、鸡四类动物的心脏、肝脏、肺脏中微量硒的测定方法。试样在氧弹中以富氧来进行燃烧灰化,使动物脏体中各种形式存在的硒化物被氧化或分解为硒的氧化物后,用浓碱NaOH吸收燃烧产物和用导管导出装有浓NaOH溶液中予以充分吸收,然后调节溶液的pH值至5～8,再用硒-碘化钾-结晶紫-聚乙烯醇显色体系进行测定硒的含量。硒在盐酸介质中可与I3-离子形成三元络合物,其络合物的最大吸收波长为580nm,硒的含量在0～0.25μg/ml范围内服从比耳定律,运用硫脲作为还原剂,EDTA作为掩敝剂,线性回归方程为C=0.4676A-0.0055,相关系数r为0.9995。通过对方法进行回收率测定,回收率在99.14%～100.79%,方法可用于动物脏体内微量硒的测定。

关键词: 硒, 氧弹燃烧, 分光光度法, 动物脏体

Abstract: The determining method of trace selenium in animal viscera was studied by combining of oxygen bomb ash test and spectrophotometry. The samples of animal viscera were decomposed by burning with abundant oxygen in oxygen bomb. Then the selenium compound in different forms of food was either oxidized or decomposed to oxides or free selenium vapor, the decomposed product were fully absorbed by thick base liquid NaOH and then the pH value of absorbed liquid set to 5～8. In HCl a ternary complex solution was formed among Se(IV) I3- and crystal violet in the presence of PAV. The absorption maximum is 580nm. The concentration range of 0～0.25μg/ml of selenium obeys beer’s law. By separation on EDTA as shelter solvent and thiourea as reductive, the interference of most of the coexisting elements was eliminated. Equation of linear regression equation is C=0.4676A-0.0055, the related coefficient r is 0.9995 and the recovery rates were the range of 99.14%～100.79%. This method has been applied to the determination of trace selenium in animal internal organs with satisfactory results.

Key words: selenium, oxygen bomb combustion, animal internal organs

粟智, 车云霞, 高学平, 申泮文. 动物脏体中微量硒的测定[J]. 食品科学, 2006, 27(4): 202-205.

SU Zhi, CHE Yun-Xia, GAO Xue-Ping, SHEN Pan-Wen. Determination of Trace Selenium in Animal Viscera[J]. FOOD SCIENCE, 2006, 27(4): 202-205.

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