沙蒿籽胶多糖的分级纯化和结构分析

食品科学 ›› 2007, Vol. 28 ›› Issue (3): 73-76.

沙蒿籽胶多糖的分级纯化和结构分析

刘敦华, 谷文英

宁夏大学农学院；江南大学食品学院宁夏银川750011；江苏无锡214036；

出版日期:2007-03-15 发布日期:2011-12-31

Fractionation and Structure of Polysaccharides of Artemisia sphaerocephala Krasch gum

LIU Dun-Hua, GU Wen-Ying

1.Agricultural College, Ningxia University, Yinchuan 750011, China; 2.School of Food Science and Technology, Southern Yangtze University, Wuxi 214036, China

Online:2007-03-15 Published:2011-12-31

摘要/Abstract

摘要： 采用0.1mol/L的氢氧化钾对沙蒿籽胶多糖进行组分分级得到水溶多糖(AG1)和水不溶多糖(AG2)。利用离子交换柱层析从AG1中分离得到酸性多糖(AG11)和中性多糖(AG12)。经凝胶过滤色谱检测,AG11和AG12为均一组分。化学组成分析表明,AG1为葡萄糖糖基组成的葡聚糖,并含有糖醛酸;AG11和AG12分别为阿拉伯糖、鼠李糖、木糖、甘露糖、葡萄糖和半乳糖等糖基组成的杂多糖,并含有糖醛酸,相对分子质量分别为8.63×105Da和3.46×105Da。红外光谱测定表明,AG11、AG12和AG2具有多糖的特征吸收,并且其糖环均为吡喃环。

关键词: 沙蒿籽胶, 分级, 纯化, 结构

Abstract: The polysaccharides of Artemisia sphaerocephala Krasch gum were graded into water-soluble polysaccharide (AG1) and water-insoluble polysaccharide (AG2) with 0.1mol/L NaOH AG11 and AG12 were obtained with ion-exchange method from AG1, shown to be homogeneous by gel filtration chromatography. AG2 contains glucose predominantly, and also uronic acid. AG11 and AG12 contains arabinose, rhamnose, xylose, mannose, glucose and galactose predominantly and respectively, and also uronic acid. The molecular weights of AG11 and AG12 are 8.63×105Da and 3.46×105Da respectively. The structure of AG11, AG12 and AG2 contains pyranride and has the characteristic of glycoconjugate. The polysaccharides of Artemisia sphaerocephala Krasch gum were graded into water-soluble polysaccharide (AG1) and water-insoluble polysaccharide (AG2) with 0.1mol/L NaOH AG11 and AG12 were obtained with ion-exchange method from AG1, shown to be homogeneous by gel filtration chromatography. AG2 contains glucose predominantly, and also uronic acid. AG11 and AG12 contains arabinose, rhamnose, xylose, mannose, glucose and galactose predominantly and respectively, and also uronic acid. The molecular weights of AG11 and AG12 are 8.63×105Da and 3.46×105Da respectively. The structure of AG11, AG12 and AG2 contains pyranride and has the characteristic of glycoconjugate.

Key words: Artemisia sphaerocephala Krasch gum, fractionation, purification, structure

刘敦华, 谷文英. 沙蒿籽胶多糖的分级纯化和结构分析[J]. 食品科学, 2007, 28(3): 73-76.

LIU Dun-Hua, GU Wen-Ying. Fractionation and Structure of Polysaccharides of Artemisia sphaerocephala Krasch gum[J]. FOOD SCIENCE, 2007, 28(3): 73-76.

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