食品科学 ›› 2007, Vol. 28 ›› Issue (6): 127-131.

• 工艺技术 • 上一篇    下一篇

脉冲超声波同时提取紫菜中蛋白和多糖的工艺研究

 肖海芳, 马海乐, 朱文学   

  1. 河南科技大学食品与生物工程学院; 河南科技大学食品与生物工程学院 河南洛阳471003; 河南洛阳471003;
  • 出版日期:2007-06-15 发布日期:2011-12-31

Simultaneous Extraction of Protein and Polysaccharide from Porphyra yezoensis by Pulsed Ultrasound

 XIAO  Hai-Fang, MA  Hai-Le, ZHU  Wen-Xue   

  1. Food and Bioengineering Department, Henan University of Science and Technology, Luoyang 471003, China
  • Online:2007-06-15 Published:2011-12-31

摘要: 以条斑紫菜为原料,采用脉冲超声波同时提取蛋白和多糖。通过单因素试验和正交试验研究了不同因素对提取效果的影响。结果表明,提取全程时间、超声功率、提取温度、料液比对提取效果影响显著,最优工艺参数为搅拌速度13r/s、超声发出时间1.5s、超声间隙时间2s、提取全程时间85min、超声功率720W、提取温度30℃和料液比15mg/ml,在此条件下,多糖提取得率达35.065%,蛋白提取得率达45.728%。

关键词: 条斑紫菜, 脉冲超声波, 提取, 蛋白, 多糖

Abstract: Simultaneous extraction of protein and polysaccharide from Porphyra yezoensis by pulsed ultrasound was studied. By single factor and orthogonal test the optimum conditions are as follows: pump revolving speed 13 r/s, ultrasonic running time in a circle 1.5 s, ultrasonic interim time in a circle 2 s, ultrasonic power 720 W, extraction time 85 min, temperature 35 ℃ and material/solution ratio 15 mg/ml. By the above conditions, the extraction yield of polysaccharide is 35.065%, and the extraction yield of protein 45.728%.

Key words: Porphyra yezoensis, pulsed ultrasound, extraction, protein, polysaccharide