一氧化氮对采后油菜品质与活性氧代谢相关酶的影响

食品科学 ›› 2007, Vol. 28 ›› Issue (7): 493-496.

一氧化氮对采后油菜品质与活性氧代谢相关酶的影响

陈海荣, 申琳, 欧阳丽喆, 范蓓, 刘灿, 孙建军, 生吉萍

中国农业大学食品科学与营养工程学院；山东省菏泽信息工程学校；中国农业大学食品科学与营养工程学院北京100083；北京100083；山东菏泽274006；

出版日期:2007-07-15 发布日期:2011-10-24

Effects of Nitric Oxide (NO) on Postharvest Rape Quality and Reactive Oxygen Specious (ROS) Metabolism Related Enzymes

CHEN Hai-Rong, SHEN Lin, 欧Yang-Li-Zhe , FAN Bei, LIU Can, SUN Jian-Jun, SHENG Ji-Ping

1.College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; 2.School of Message Engineering of Heze, Heze 274006, China

Online:2007-07-15 Published:2011-10-24

摘要/Abstract

摘要： NO是植物体内重要的信号分子,其在采后保鲜方面的应用是近年研究的焦点。本研究以油菜(Brassicarapa L.ssp.chinensis,cv.Huaguan)为试材,使用一氧化氮(NO)供体100μmol/L硝普钠(SNP)-0.04mp真空渗透处理2min,贮藏于25℃相对湿度(RH)70%的环境中。在贮藏后0、1、2、3、4d分别测定油菜的腐烂率及黄变率;并测定贮藏过程中超氧化物歧化酶(SOD),过氧化氢酶(CAT),抗坏血酸过氧化物酶(APX)和谷胱甘肽还原酶(GR)的活性。结果表明,SNP处理显著降低了采后油菜的黄变率及腐烂率;提高了油菜贮藏期间SOD、APX的活性,降低了1～3d的GR活性;贮藏1d时提高了CAT的活性,2d之后抑制CAT活性。说明100μmol/L SNP处理提高了油菜采后品质,并可能通过提高贮藏期前期CAT活性和整个贮藏期间SOD及APX的活性延缓油菜采后品质的下降。

关键词: 油菜, 一氧化氮, 采后品质, 活性氧代谢相关酶

Abstract: In this study, rape (Brassica rapa L. ssp. chinensis, cv. Huaguan) was treated with 100 μmol/L sodium nitroprusside (SNP, a nitric oxide donor) solution treated, by vacuum infiltration for 2 min, and stored at 25 ℃ with RH 70% for 4 d. On 0, 1, 2, 3, 4 d, the postharvest quality indicators including yellowing rate and decay rate were determined; and the activity of reactive oxygen species (ROS) metabolism related enzymes, including superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and glutathione reductase (GR). The results showed that the yellowing rate and decay rate of SNP treated group are significantly lowered better than that of control. SNP treatment makes the SOD and APX activities of rape increase during the whole storage time, but the GR activity decreases from 1 d to 3 d. Moreover, SNP treatment makes the CAT activity increase on the first day, but decrease after the second day. It showed that 100 μmol/L NO donor, SNP, significantly improves postharvest rape quality, and this effect is mightily promoting CAT activity in the beginning of the storage and promoting SOD and APX activities during storage time.

Key words: pakchoi, nitric oxide, postharvest quality, ROS metabolism related enzymes

陈海荣, 申琳, 欧阳丽喆, 范蓓, 刘灿, 孙建军, 生吉萍. 一氧化氮对采后油菜品质与活性氧代谢相关酶的影响[J]. 食品科学, 2007, 28(7): 493-496.

CHEN Hai-Rong, SHEN Lin, 欧Yang-Li-Zhe , FAN Bei, LIU Can, SUN Jian-Jun, SHENG Ji-Ping. Effects of Nitric Oxide (NO) on Postharvest Rape Quality and Reactive Oxygen Specious (ROS) Metabolism Related Enzymes[J]. FOOD SCIENCE, 2007, 28(7): 493-496.

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