食品科学 ›› 2007, Vol. 28 ›› Issue (9): 165-168.

• 工艺技术 • 上一篇    下一篇

微波辅助提取红车轴草异黄酮的工艺研究

 金汝城, 赵国磊, 李贵文, 逄艳   

  1. 兰州理工大学生命科学与工程学院; 兰州理工大学石油化工学院; 深圳大学应用化学化工学院 甘肃兰州730050; 甘肃兰州730050; 广东深圳518060;
  • 出版日期:2007-09-15 发布日期:2011-11-22

Study on Microware-assisted Extraction of Isoflavones in Tifolium pratense

 JIN  Ru-Cheng, ZHAO  Guo-Lei, LI  Gui-Wen, PANG  Yan   

  1. 1.College of Life Science and Engineering,Lanzhou University of Technology,Lanzhou 730050,China;2.College of Petrochemical Technology,Lanzhou University of Technology,Lanzhou 730050,China;3.College of Chemical and Technology,University of Shenzhen,Shenzhen 518060,China
  • Online:2007-09-15 Published:2011-11-22

摘要: 采用密闭微波提取装置对红车轴草异黄酮成分进行提取工艺的研究。通过单因素和正交试验对红车轴草异黄酮提取工艺中乙醇浓度、微波功率、料液比、提取时间和提取次数等影响异黄酮提取率的因素进行探讨。红车轴草异黄酮的最佳提取工艺参数为:乙醇浓度70%,微波功率高火(750W),料液比1:15,提取时间1min,提取三次,异黄酮提取率可达到98.91%。与超声波提取、乙醇回流提取和索氏提取相比,微波提取具有提取速度快,选择性好及操作方便等特点。

关键词: 微波提取, 红车轴草, 异黄酮, 正交试验

Abstract: Extraction technology of isflavones in Trifolium pratense was developed by using an obturated microwave-assisted extraction(MAE)equipment.Effects of ethanol-water solvent,microwave power,raito of liquid to material,extraction time and extraction times on extraction rate of isflavones were investigated by single factor and orthogonal test.The optimum extraction conditions for Trifolium pratense isflavones were established as follows:70% ethanol(V/V)as solvent,ratio of liquid to material 15:1(V/W),microwave power high fire(750W),extraction for 3 times and 1 min for each time.The extraction rate of isflavones in Trifolium pratense was as high as 98.91%.Compared with ultrasonic extraction,refluence extraction and Soxhlet extraction,microwave extraction is a rapid method with a good selectivity and handle expediently.

Key words: microwave extraction, Trifolium pratense, isoflavone, orthogonal test