食品科学 ›› 2007, Vol. 28 ›› Issue (9): 360-363.

• 生物工程 • 上一篇    下一篇

乳蛋白水解产生肽工艺条件的优化

 江小云, 潘道东   

  1. 南京师范大学食品科学与营养系; 南京师范大学食品科学与营养系 江苏南京210097; 江苏南京210097;
  • 出版日期:2007-09-15 发布日期:2011-11-22

Study on Optimum Technologic Conditions of Hydrolyzation of Milk Protein for Peptides Production

 JIANG  Xiao-Yun, PAN  Dao-Dong   

  1. Department of Food Science and Nutrition,Nanjing Normal University,Nanjing 210097,China
  • Online:2007-09-15 Published:2011-11-22

摘要: 以肽含量为指标,确定了酶添加量及复合酶(胰凝乳蛋白酶、胰蛋白酶)的酶比,且分别考察了奶粉浓度、温度、pH、反应时间对酶解乳蛋白产生肽的影响。通过正交试验确定了复合酶酶解乳蛋白的最佳工艺条件:加酶量2500U/ml,胰凝乳蛋白酶:胰蛋白酶=1:3,奶粉浓度25%(W/V),温度45℃,pH8.0,水解时间2h。在此条件下,肽浓度可达1.82mg/ml。

关键词: 乳蛋白, 酶解,

Abstract: Milk protein was hydrolyzed into peptides by enzyme in this research.Based on the output of peptides,the main factors that affect the hydrolyzation were experimentally studied in details.The results indicated that the output of the peptides could reach 1.82 mg/ml under the optimal conditions of the hydrolyzation temperature 45 ℃,the hydrolyzation time 2 h,the skim milk powder concentration 25%,the chymotrypsin-trypsin ratio 1:3,the complex enzyme added amount 2500 U/g,and the solution pH 8.0.

Key words: milk protein, hydrolysis, peptide