食品科学 ›› 2008, Vol. 29 ›› Issue (1): 111-114.

• 基础研究 • 上一篇    下一篇

液相体系制备大豆酸沉蛋白-葡聚糖共价复合物及其反应机制(Ⅰ)共价复合物的制备

 齐军茹, 杨晓泉, 廖劲松   

  1. 华南理工大学食物蛋白工程研究中心; 华南师范大学生命科学学院 广东广州510640; 广东广州510640; 广东广州510631;
  • 出版日期:2008-01-15 发布日期:2011-07-28

Preparation and Theoretical Analysis of Soy Protein-polysaccharide Conjugates Obtained in Liquid System (Ⅰ) Preparation of Soy Protein-dextran Conjugates

 QI  Jun-Ru, YANG  Xiao-Quan, LIAO  Jin-Song   

  1. 1.Food Protein Engineering Research Center, South China University of Technology, Guangzhou 510640, China; 2.College of Life Science, South China Normal University, Guangzhou 510631, China
  • Online:2008-01-15 Published:2011-07-28

摘要: 作为一项新的有效的制备方法,对液相体系中发生Maillard反应的的各种影响因素进行系统研究,结果表明,80%乙醇体系中,反应体浓度为60%、50℃时反应6h以及95%乙醇体系中,反应体浓度为60%、60℃时反应24h条件下得到的产物表现出了优越的乳化活性。并采用SDS-PAGE技术证实蛋白与多糖之间发生共价结合反应。

关键词: 共价复合物, 大豆酸沉蛋白, 乳化活性, Maillard反应

Abstract: As a new and efficient method, various factors affecting the Maillard reaction in liquid system were studied in a series of experiments. It showed that products having the same excellent emulsifying properties with 80% ethanol reacting system, concentration of reactant is 60%, 50 ℃ during 6 h with 95% ethanol reacting system while the concentration of reactant is 60%, 60 ℃ during 24 h, the emulsifying properties become optimized and could be compared with dry-heating. The covalent attachment of polysaccharides to protein was confirmed by SDS-PAGE.

Key words: conjugate, SAPP, emulsifying properties, maillard reaction