食品科学 ›› 2019, Vol. 40 ›› Issue (12): 46-54.doi: 10.7506/spkx1002-6630-20180515-216

• 食品化学 • 上一篇    下一篇

发芽糙米蛋白-葡聚糖共价接枝复合物的开发及其物理化学性能、乳液稳定性的评价

王 雪1,2,侯占群3,苑 鹏3,柳 嘉3,段盛林3,孙爱东1,*   

  1. 1.北京林业大学生物科学与技术学院,北京 100083;2.黑龙江飞鹤乳业有限公司,北京 100015;3.中国食品发酵工业研究院有限公司,北京 100015
  • 出版日期:2019-06-25 发布日期:2019-06-28
  • 基金资助:
    “十三五”国家重点研发计划重点专项(2018YFD0400900);国家自然科学基金面上项目(31871817;31471593);国家自然科学基金青年科学基金项目(31501526)

Development of Germinated Brown Rice Protein and Dextran Conjugate as an Emulsifier: Evaluation of Physicochemical Properties and Emulsion Stability

WANG Xue1,2, HOU Zhanqun3, YUAN Peng3, LIU Jia3, DUAN Shenglin3, SUN Aidong1,*   

  1. 1. College of Biological Sciences & Technology, Beijing Forsetry University, Beijing 100083, China; 2. Heilongjiang Feihe Dairy Co. Ltd., Beijing 100015, China; 3. China National Research Institute of Food & Fermentation Industries Co. Ltd., Beijing 100015, China
  • Online:2019-06-25 Published:2019-06-28

摘要: 将发芽糙米蛋白(germinated brown rice protein,GBRP)和葡聚糖通过美拉德反应开发一种新型的乳化剂。使用傅里叶变换红外光谱仪和X射线衍射仪分析发芽糙米蛋白-葡聚糖共价复合物(germinated brown rice protein-dextran conjugate,GBRP-Dex Con)。结果表明,通过与葡聚糖共价结合,相比于GBRP,GBRP-Dex Con的溶解度提高了10%(pH 7.0);变性温度(122.2 ℃)提高了14.7 ℃(GBRP为107.5 ℃);GBRP-Dex Con清除DPPH自由基能力及总还原能力显著提高(P<0.05)。通过测定美藤果油乳状液的粒径及物理稳定性发现,GBRPDex Con在稳定乳状液比GBRP、发芽糙米蛋白-葡聚糖混合物(GBRP-Dex Mix)以及作为常规乳化剂的大豆分离蛋白更为有效。本方法制备的乳化剂GBRP-Dex Con具有优异的性能和应用到食品工业的潜力。

关键词: 发芽糙米蛋白, 葡聚糖, 共价复合物, 粒径, 物理化学稳定性

Abstract: The purpose of this study was to develop a novel emulsifier from germinated brown rice protein (GBRP) and dextran through the Maillard reaction. Fourier transform infrared (FTIR) spectroscopy and X-ray diffractometry analysis were used to evidence the formation of GBRP-dextran conjugate (GBRP-Dex Con). Experimental results indicated that the solubility of GBRP-Dex Con was 10% higher than that of GBRP (pH 7.0) through covalent bonding with dextran. The denaturation temperature of GBRP-Dex Con (122.2 ℃) was 14.7 ℃ higher than that of GBRP (107.5 ℃). The radical scavenging activity and reducing power of GBRP-Dex Con were significantly higher than those of GBRP (P < 0.05). Measurements of the droplet size and physical stability of Sacha Inchi oil emulsion demonstrated that GBRP-Dex Con was more effective in stabilization of the emulsion than the protein alone, their physical mixture and the common emulsifier soy protein isolate (SPI). Thus, GBRP-Dex Con as a new emulsifier with excellent properties has a great potential for applications in the food industry.

Key words: germinated brown rice protein (GBRP), dextran, conjugate, droplet size, physicochemical stability

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