食品科学 ›› 2008, Vol. 29 ›› Issue (1): 129-133.

• 工艺技术 • 上一篇    下一篇

富硒菜籽蛋白肽的精制研究

 刘大川, 周俊梅   

  1. 武汉工业学院; 国家粮食储备局武汉科学研究设计院 湖北武汉430023; 湖北武汉430079;
  • 出版日期:2008-01-15 发布日期:2011-07-28

Study on Refining of Selenium-enriched Rapeseed Protein Peptides

 LIU  Da-Chuan, ZHOU  Jun-Mei   

  1. 1.Wuhan Polytechnic University, Wuhan 430023, China; 2.Institute of Wuhan Scientific Research and Design, State Administration for Grain Reserve, Wuhan 430079, China
  • Online:2008-01-15 Published:2011-07-28

摘要: 对富硒菜籽分离蛋白酶水解得到的水解液粗肽产品进行精制,活性炭脱色脱苦,离子交换和透析脱盐处理。脱色工艺在单因素试验的基础上,通过正交试验优化条件为:活性炭用量1.25%,pH3.0,脱色时间60min,温度70℃。两种脱盐方法的脱盐率都很高,均达到85%以上。精制后的产品其平均分子量在1500D以下,是小分子多肽。

关键词: 富硒菜籽蛋白肽, 脱色, 脱盐, 分子量

Abstract: Selenium-enriched rapeseed protein peptides were prepared by enzyme-hydrolyzing protein isolates. The crude peptide was refined by discoloring with active carbon and desalination. The single factor tests and orthogonal tests have been applied to optimize the conditions of discolouration as follows: active carbon concentration 1.25%, pH3.0, time 60 min and temperature 70 ℃. The effects of desalination are evident and the desalting efficiency is higher than 85%. The refined peptide produced has lower 1500 D average molecular weight.

Key words: Selenium-enriched rapeseed protein peptides, discolouration, desalination, molecular weight