食品科学 ›› 2008, Vol. 29 ›› Issue (1): 141-145.

• 工艺技术 • 上一篇    下一篇

二次回归正交旋转组合设计对麦麸中阿拉伯木聚糖酶解工艺的优化

 张晓娜, 周素梅, 王世平   

  1. 中国农业科学院农产品加工研究所; 中国农业大学食品科学与营养工程学院 北京100094中国农业大学食品科学与营养工程学院; 北京100083; 北京100094中国农业大学食品科学与营养工程学院;
  • 出版日期:2008-01-15 发布日期:2011-07-28

Optimizing Enzymatic Hydrolysis Conditions of Arabinoxylan in Wheat Bran through Quadratic Orthogonal Rotation Combination Desig

 ZHANG  Xiao-Na, ZHOU  Su-Mei, WANG  Shi-Ping   

  1. 1.Institute of Processing for Agricultural Products, Chinese Academy of Agricultural Sciences, Beijing 100094, China; 2.College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
  • Online:2008-01-15 Published:2011-07-28

摘要: 在小麦麸皮中阿拉伯木聚糖酶解工艺初步研究的基础上,采用二次回归正交旋转组合设计对其酶解工艺进行了优化。建立了水溶性阿拉伯木聚糖(WEAX)得率(Y)对温度(X1)、时间(X2)、酶用量(酶与底物质量比,g/g)(X3)及pH值(X4)四个试验因素的正交回归模型:Y=12.84921-1.36983X1+0.69076X2+0.75443X3-1.46276X12-0.93773X42。从模型推知,当温度为60℃、时间为3h、酶用量为0.75%、pH值为5时,得率最大值达到15.28%,验证实验结果与模型值相符。

关键词: 阿拉伯木聚糖, 戊聚糖酶, 酶解工艺, 正交旋转组合设计, 优化

Abstract: Based on the primary studies on the enzymatic hydrolysis conditions of arabinoxylan in wheat bran, the enzymatic degradation conditions of arabinoxylan were optimized by quadratic regression orthogonal rotary tests. The quadratic orthogo-nal regression model is of arabinoxylan yield (Y) to four factors of temperature (X1), time (X2), the concentration of enzyme (X3) and pH value (X4) and established as Y=12.84921-1.36983X1+0.69076X2+0.75443X3-1.46276X12-0.93773X42. It was concluded from the model that when temperature, time, enzyme concentration and pH value are 60 ℃, 3 h, 0.75% and 5 respectively, the yield (Y) is the maximal (15.28%) and consistent with the confirmatory experiment result.

Key words: arabinoxylan, pentosanase, enzymatic hydrolysis conditions, orthogonal rotation combination design, optimization