食品科学 ›› 2008, Vol. 29 ›› Issue (1): 207-211.

• 生物工程 • 上一篇    下一篇

高产纤溶酶霉菌固体发酵工艺条件的优化

 闵伟红, 李佳, 王影, 张锦玉, 李玉   

  1. 吉林农业大学食品工程学院; 长春职业技术学院食品与生物技术分院; 吉林农业大学菌物研究所 吉林长春130118; 吉林长春130118; 吉林长春130033;
  • 出版日期:2008-01-15 发布日期:2011-07-28

Solid Fermentation Optimizations of Plasmin High-producing Mold  

 MIN  Wei-Hong, LI  Jia, WANG  Ying, ZHANG  Jin-Yu, LI  Yu   

  1. 1.College of Food Engineering, Jilin Agricultural University, Changchun 130118, China; 2.Mycological Institute, Jilin Agricultural University, Changchun 130118, China; 3.School of Food and Biological Technology, Changchun Vocational Institute of Technology, Changchun 130022, China
  • Online:2008-01-15 Published:2011-07-28

摘要: 从我国传统发酵豆制品中筛选出一株具有纤溶酶活性的霉菌菌株,初步鉴定为枯青霉,经诱变后纤溶酶活力为660U/g。本实验以其为出发菌株,对该菌株固体发酵条件进行优化,结果表明菌株产酶最佳条件为:m(麸皮):m(豆粕)=1:3,自然pH值,加水量0.75ml/g物料,MnSO4·H2O和(NH4)2SO4加量分别为0.25%和1.4%,接种量17.5%,发酵温度30℃,发酵时间72h,在该条件下固体培养纤溶酶活性平均达到873.76U/g,比优化前提高了213U/g。

关键词: 纤溶酶, 固体发酵, 优化

Abstract: A plasmin activity producing mold was screened from traditional bean ferment products. The mold is found to be Penicillium curinum Thom by appraisement. The plasmin activity reachs 660 U/g after mutagenesis. The optimized ferment conditions for the mold are: m(wheat bran):m(pea)=1:3, pH 5.5, 0.75 ml water per 1 g materials, MnSO·4H2O 0.25%, (NH4)2SO4 1.4%, innoculation quantity 17.5%, fermentation temperature 30 ℃ and fermentation time 72 h. Under these conditions, the mold plasmin activity is originally 873.76 U/g whereas it is increased to 213 U/g after being optimized.

Key words: fibrinolysin, solid fermentation, optimization