关键词: 嗜酸乳杆菌L101, 增殖因子, 单因素试验, 正交试验

Abstract: In order to promote the growth of Lactobacillus acidophilusL 101 (LAL101),six growth promoters (tomato juice,carrot juice,cap fungus juice,soybean sprout juice,beer and isomaltooligosaccharide) were selected and applied to basal medium (MRS) with different ratios. The effect of each promoter on the growth of LAL101was evaluated in single factor tests and the optimal combination of these growth promoters were optimized by orthogonal tests. The results showed that when LAL101 is incubated at 38 ℃ for a period of 14 h under non-pH-controlled condition,the better growth of LAL101 is observed in basal medium supplemented with 10% (V/V) soybean sprout juice,which shows the best promotion effects among the selected ones,with the viable count being 4.034×109 CFU/ml. The maximum growth of LAL101 is observed,when basal medium is supplemented with the best combination of four growth promoters,soybean sprout juice 5% (V/V),cap fungus juice 10% (V/V),tomato juice 10% (V/V) and isomaltooligosacharide(10g/L),and when LAL101 is incubated at 38℃ for a period of 14 h under non-pH-controlled condition,the viable count reaches 5.25×1010 CFU/ml. The results of this study can provide the necessary technological parameters for the growth of LAL101 in production.

Key words: Lactobacillus acidophilus L101, growth promoter, single factor test, orthogonal test