食品科学 ›› 2008, Vol. 29 ›› Issue (12): 185-189.

• 工艺技术 • 上一篇    下一篇

响应面法优化酶解制备板栗清汁工艺条件研究

 但晓容, 张颖, 卢晓黎   

  1. 四川大学食品工程系;
  • 出版日期:2008-12-15 发布日期:2011-12-08

Study on Optimization of Hydrolysis Process Parameters of Chinese Chestnut for Preparing Beverage with Response Surface Methodology

 DAN  Xiao-Rong, ZHANG  Ying, LU  Xiao-Li   

  1. Department of Food Engineering,Sichuan University,Chengdu 610065,China
  • Online:2008-12-15 Published:2011-12-08

摘要: 运用响应面法,对酶解制备板栗清汁条件进行优化,得出液化优化参数为:料液比1:4.21,酶添加量25.04U/g,酶解时间39.83min,pH5.54,在此条件下板栗清汁的透光率76.25%,吸光度0.30,可溶性固形物含量2.78%,最佳综合指标为1.88;得出糖化优化参数为:酶添加量80.44U/g,酶解温度62.01℃,酶解时间4.21h,pH5.68,在此条件下板栗清汁的透光率79.69%,吸光度0.26,可溶性固形物含量6.26%,最佳综合指标为4.34。

关键词: 板栗清汁, 酶解条件, 响应面法

Abstract: The liquefaction and saccharification process parameters of Chinese chestnut beverage were optimized by using response surface methodology. The optimal liquefaction process parameters are ratio of solid to liquid 1:4.21,α-amylase amount 25.04 U/g,hydrolysis time 39.83 min and pH 5.54. Under these conditions,the penetrability of the obtained Chinese chestnut hydroly sate is 76.25%,the chromaticity is 0.30,the solid content is 2.78%,and the best synthetic index is 1.88. And the optimal process saccharification parameters are glucoamylase amount 80.44 U/g,hydrolysis time 4.21 h,saccharification temperature 62.01℃ and pH 5.68. Under these conditions,the penetrability of the final hydrolysate is 79.69%,the chromaticity is 0.26,the solid content is 6.26%,and the best synthetic index is 4.34.

Key words: Chinese chestnut beverage, hydrolysis condition, response surface methodology(RSM)