食品科学 ›› 2008, Vol. 29 ›› Issue (2): 425-429.

• 包装贮运 • 上一篇    下一篇

几种高安全性生物抗菌成分对淡水鱼腌制保鲜特性的研究

 吴正奇   

  1. 湖北工业大学生物工程学院 湖北武汉430068;
  • 出版日期:2008-02-15 发布日期:2011-08-24

Study on Anti-microbial Activities of Several High Safety Biological-preservatives on Fresh Water Fish

 WU  Zheng-Qi   

  1. College of Bioengineering, Hubei University of Technology, Wuhan 430068, China
  • Online:2008-02-15 Published:2011-08-24

摘要: 本实验采用平板菌落记数法,研究了鱼精蛋白、富马酸、甘氨酸等10余种具有保鲜作用的生物成分对淡水鱼(白鲢鱼)中微生物的抑制效果,测出其半抑菌浓度(IC50)分别为:葡萄糖酸内酯IC50=0.205%、溶菌酶IC50=0.151%、鱼精蛋白IC50=0.305%、富马酸IC50=0.431%、富马酸钠IC50=0.872%、富马酸与富马酸钠(1:1混合物)IC50=0.631%、甘氨酸IC50=0.172%、山梨酸IC50=0.145%、山梨酸钾IC50=0.497%和乳酸链球菌酶素IC50=0.219%。结果表明,从抗菌性能和成本综合考虑,葡萄糖酸内酯、甘氨酸、富马酸和山梨酸可望作为保鲜成分用于淡水鱼的加工过程。

关键词: 淡水鱼, 防腐保鲜, 半抑菌浓度(IC50)

Abstract: In this experiment, the plate count method was used to detect the inhibit effects of around 10 biological preservatives, such as gluconolactone, fumaric acid, glycine and others against the microorganisms of the fresh water fish. The results showed that each half inhibit concentration(IC50) is respectively:gluconolactone IC50 0.205%, lysozyme IC50 0.151%, protanmine IC50 0.305%, fumaric acid IC50 0.431%, sodium fumaric acid IC50 0.872%, the glycine IC50 0.172%, potassium sorbic IC50 0.497%, sorbic acid IC50 0.145%, nisin IC50 0.219%. For the sake of considering the cost with the inhibit effects, gluconolactone, glycine and fumaric acid are chosen as anti-microbial agents in processing fresh water fish.

Key words: fresh water fish, anti-microorganism, half inhibit concentration(IC50)