关键词: 树舌, 菌丝体, 碳源, 氮源, 转速

Abstract: Ganederma applantatum is a species of the Ganoderma genus; it is one of the precious medicinal mushroom in the treasury of Chinese traditional medicines. To study the high yield and economical medium productive conditions, this experiment selected and used single factors such as rational speed, temperature, pH value, bottled volume, inoculated bulk, carbon source, nitrogen source and C/N ratio as multifactor tests with the submerge culture of the mycelia to explore the effects of these various factors on the mycelial yield. The results of this experiment indicated that the optimal rotational speed for the growth of mycelia is 150 r/min, temperature 25～30 ℃, pH value 5.5～6.5, bottled volume 125 ml, and inoculated bulk 5 ml/100 ml. Meanwhile glucose is the optimum carbon source, rice bran the nitrogen source, and the C/N ratio 25:1. The optimal nutritional conditions when the mycelia of this sort of fungi were cultivated by means of submerged culture are as follow: 3% glucose, 0.072% KNO3, 0.02% NaH2PO4 and pH 8.5. Through the growth curve test the logistic equation is deduced: Y=0.616/(1+17.732e-8.874x). The weight of the dry mycelia reaches 500～700 mg/100 ml.

Key words: Ganoderma applanatum, mycelium, carbon sources, nitrogen sources, rotate speed