食品科学 ›› 2008, Vol. 29 ›› Issue (6): 159-163.

• 工艺技术 • 上一篇    下一篇

预处理猪血清IgG料液的超滤浓缩研究

 罗磊, 樊金铃, 孙红姣   

  1. 河南科技大学食品与生物工程学院; 南阳市土壤肥料工作站
  • 出版日期:2008-06-15 发布日期:2011-08-26

Study on Concentration of Porcine Serum IgG Solution by Ultrafiltration

 LUO  Lei, FAN  Jin-Ling, SUN  Hong-Jiao   

  1. 1. College of Food and Bioengineering, Henan University of Science and Technology; 2. Soil and Fertilizer Workstation of Nanyang
  • Online:2008-06-15 Published:2011-08-26

摘要: 本实验对预处理猪血清IgG料液超滤浓缩进行了研究。选择螺旋卷式超滤设备,以截留液全循环错流方式对预处理IgG料液进行超滤浓缩。其最佳工艺参数为:样品pH值为7、温度50℃、超滤压力0.1MPa、浓缩倍数为10倍。浓缩后得到的样品中蛋白质含量为2.08%(W/V),IgG含量为1.42%(W/V),具有活性的IgG所占比例比超滤前略有下降,从91.3%降低到87.6%。

关键词: 猪血清, G型免疫球蛋白, 超滤浓缩

Abstract: This research aimed to concentrate the porcine serum IgG solution by spire roll ultrafiltration membrane module, with all retention circulates crosscurrent flow mode. The optimal technological parameters of ultrafiltration concentration are determined as follows: sample solution pH 7, temperature 50 ℃, ultrafiltration pressure 0.1 MPa and concentration multiple 10. Total protein content in concentrated sample 2.08% (W/V). IgG content in concentrated sample is 1.42% (W/V), and the proportion of active IgG reduces from 91.3% (W/V) to 87.6% (W/V).

Key words: porcine serum, immunoglobulin G (IgG), ultrafiltration concentration