毛竹竹叶多糖分离与纯化技术研究

食品科学 ›› 2008, Vol. 29 ›› Issue (7): 156-159.

毛竹竹叶多糖分离与纯化技术研究

周跃斌,王伟,周向荣,李适

湖南农业大学产业处；湖南农业大学天然产物研究中心；湖南省质量技术监督局标准化研究院；湖南农业大学天然产物研究中心湖南长沙410128；湖南长沙410128；湖南长沙410007

出版日期:2008-07-15 发布日期:2011-07-28

Study on Isolation and Purification Technics of Polysaccharides Extracted from Leaves of Phyllostachys pubescens

ZHOU Yue-Bin, WANG Wei, ZHOU Xiang-Rong, LI Shi

1.Product Agent, Hunan Agricultural University, Changsha 410128, China; 2.Research Center for Natural Products, Hunan Agricultural University, Changsha 410128, China; (3. Institute of Standardization, Hunan Bureau of Quality and Technology Supervision, Changsha 410007, China

Online:2008-07-15 Published:2011-07-28

摘要/Abstract

摘要： 对毛竹叶采用超声波提取法获得的竹叶多糖粗提取物,采用Sevag法脱蛋白质、乙醇分级沉淀、冷冻干燥后得到粗多糖,进一步用DEAE-52纤维素柱层析、Sephadex葡聚糖凝胶柱层析等方法分离纯化。结果表明:Sevag法脱蛋白3次可脱除97.2%的蛋白质;50%浓度的乙醇沉淀多糖得率最高,为37.6%;DEAE-52纤维素柱层析柱水洗脱的得率最高,为46.4%;DEAE-52纤维素柱层析柱2次水洗脱获得的竹叶多糖经G-75柱层析和G-100柱层析可得到白色粉末状的中性多糖。选择水和NaCl溶液为洗脱剂的温和条件分离纯化多糖效果较好。

关键词: 毛竹, 竹叶多糖, 柱层析, 分离, 纯化

Abstract: Polysaccharides were prepared from the leaves of Phyllostachys pubescens. The crude polysaccharides were extracted from the dried leaves by ultrasonic method and fractionated precipitation by 95% ethanol while the protein was removed with Savag’s method. After being further lyophilized, these polysaccharides were purified by DEAE-52 cellulose column chromatog-raphy and Sephadex glucan gel column chromatography, respectively. The results showed that Savag’s deprotein method can get rid of 97.2% protein with the yield of polysaccharide as high as 37.6% with 50% ethanol precipitation. The yield of polysaccharides is the highest by DEAE-52 cellulose column chromatography which can be 46.4% and the polysaccharides extracted by DEAE-52 cellulose column chromatography with twice water elution can be changed into neutral polysaccharides of white powder by G-75 column chromatography and G-100 column chromatography. Water and NaCl as elution solvent can lead to good purifying efficiency on polysaccharides.

Key words: Phyllostachys pubescens, polysaccharides from bamboo leaves, column chromatography, isolation, purification

周跃斌, 王伟, 周向荣, 李适. 毛竹竹叶多糖分离与纯化技术研究[J]. 食品科学, 2008, 29(7): 156-159.

ZHOU Yue-Bin, WANG Wei, ZHOU Xiang-Rong, LI Shi. Study on Isolation and Purification Technics of Polysaccharides Extracted from Leaves of Phyllostachys pubescens[J]. FOOD SCIENCE, 2008, 29(7): 156-159.

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