食品科学 ›› 2008, Vol. 29 ›› Issue (8): 269-271.

• 工艺技术 • 上一篇    下一篇

玉米黄粉酶解提取浓缩蛋白工艺优化研究

李梦琴, 李运罡, 吴坤, 王志豪   

  1. 河南农业大学食品科学技术学院
  • 出版日期:2008-08-15 发布日期:2011-08-26

Optimization of Enzymatic Extraction Technology of Concentrated Protein from Corn Gluten Meal

 LI  Meng-Qin, LI  Yun-Gang, WU  Kun, WANG  Zhi-Hao   

  1. College of Food Science and Technology,Henan Agricultural University,Zhengzhou 450002,China
  • Online:2008-08-15 Published:2011-08-26

摘要: 通过单因素试验和响应曲面法,优化了采用低温α-淀粉酶酶解提取玉米黄粉浓缩蛋白的条件。所得最佳工艺为料液比1:4、酶用量36U/g玉米黄粉、温度40℃、时间90min、pH值6.2。该条件下玉米黄粉浓缩蛋白纯度达到78.96%,得率为80.13%。

关键词: 玉米黄粉, 浓缩蛋白, 酶法, 响应曲面法

Abstract: The technology of enzymatic extraction of concentrated protein from corn gluten meal(CGM) was optimized by mono-factor test and response surface methodology.The results showed that the optimum conditions are as follows:ratio of solid to liquid 1:4,enzyme amount 36 U α-amylase/g CGM,temperature 40 ℃,pH 6.2 and enzymatic hydrolysis time 90 min. Under these conditions,the purity of CGM concentrated protein reaches 78.96%,and its yield is 80.13%.

Key words: corn gluten meal, concentrated protein, enzymatic method, response surface methodology