关键词: 3, 5-二硝基水杨酸(DNS), 还原糖, 比色法, 测定条件

Abstract: Two kinds of 3,5-dinitrosalicylic acid(DNS) agents were respectively used for determination of reducing sugar content,and effects on determination results by the factors,such as amount of DNS reagent developing time,wavelength and storage time were discussed. The results showed that on the conditions of reagent amount around 1.5 ml,boiling bath time not less than 5 minutes,and standing for 30 minutes after developing,at the wavelength of 540 nm and 550 nm for reagent one and two respectively,the accurate results can be acquired.

Key words: 3,5-dinitrosalicylic acid(DNS), reducing sugar, spectrophotometry, determination factor