灰树花多糖的超滤分离及免疫活性研究

食品科学 ›› 2008, Vol. 29 ›› Issue (9): 277-280.

灰树花多糖的超滤分离及免疫活性研究

杨阳, 刘承初, 贾薇, 刘艳芳, 杨炎, 张劲松, 周昌艳

上海海洋大学食品学院；上海农业科学院食用菌研究所；

出版日期:2008-09-15 发布日期:2011-12-08

Study on Ultrafiltration Separation and Immunocompetence of Polysaccharides from Grifola frondosa

YANG Yang, LIU Cheng-Chu, JIA Wei, LIU Yan-Fang, YANG Yan, ZHANG Jin-Song, ZHOU Chang-Yan

1. College of Food Science and Technology,Shanghai Ocean University,Shanghai 200090,China; 2. Edible Fungi Institute,Shanghai Academy of Agricultural Sciences,Shanghai 201106,China

Online:2008-09-15 Published:2011-12-08

摘要/Abstract

摘要： 目的:从灰树花子实体中提取分离灰树花多糖并进行免疫活性检测。方法:热水和稀碱提取灰树花子实体多糖后,利用乙醇沉淀法和超滤法获取灰树花水溶性及碱溶性子实体多糖,通过小鼠脾淋巴细胞转化增殖实验检测免疫活性。结果:利用乙醇沉淀法获得水溶性及碱溶性多糖两个组分,得率分别为6.67%和5.88%,利用超滤法获得分子量范围分别为大于1000kD、100～1000kD以及10～100kD的共六个灰树花子实体多糖组分,其中水溶性多糖组分GFP100、GFP10和GFP1的得率分别为3.99%、0.51%和3.94%,碱溶性多糖组分GFAP100、GFAP10和GFAP1的得率分别为2.42%、2.32%和3.04%,利用超滤法获得的六个多糖组分均显示了显著的促进小鼠脾淋巴细胞转化增殖作用。结论:超滤法应用于灰树花子实体多糖的分离要优于传统乙醇沉淀法,该法工艺简便,多糖得率更高,且不损害其活性。

关键词: 灰树花多糖, 超滤, 分子量, 免疫活性

Abstract: Objective: To separate Grifola frondosa fruit body polysaccharide by ethanol precipitation and ultrofiltrationr (UF) and investigate the effects of Grifola frondosa fruit body polysaccharides on immune function of mice. Methods: Water-soluble polysaccharides were extracted from Grifola frondosa fruit body with hot water and then the residue was further extracted with dilute sodium hydroxid to obtain alkali-soluble polysaccharides. Both the two polysaccharides were precipitated out from the extracting solutions by ethanol, and they were further separated by UF according to their molecular weights (> 1000 kD, 100 to 1000 kD and 10 to 100 kD) respectively. The immunocompetences of different polysaccharide components derived from Grifola frondosa were investigated using immunocyte cultivation technique in vitro. Results: The yield of water-soluble polysaccharides is 6.67%, and the yield of alkali-soluble polysaccharides is 5.88%. The yields of water-soluble polysaccharides with molecular weight more than 1000 kD (GFP100) , 100 to 1000 kD (GFP10) and 10 to 100 kD (GFP1) are 3.99%,0.51% and 3.94%, respectively, and the yields of alkali-soluble polysaccharides with molecular weight more than 1000 kD ( GFAP100), 100 to 1000 kD (GFAP10) and 10 to 100 kD (GFAP1) are 2.42%, 2.32% and 3.04%, respectively. All the polysaccharide components separated by UF can significantly increase the proliferation rate of mouse spleen lymphocytes. Conclusion: The ultrafiltration separation of Grifola frondosa polysaccharides is simple and feasible.

Key words: Grifola frondosa polysaccharides, ultrafiltration, molecular weight, immunocompetence

杨阳, 刘承初, 贾薇, 刘艳芳, 杨炎, 张劲松, 周昌艳. 灰树花多糖的超滤分离及免疫活性研究[J]. 食品科学, 2008, 29(9): 277-280.

YANG Yang, LIU Cheng-Chu, JIA Wei, LIU Yan-Fang, YANG Yan, ZHANG Jin-Song, ZHOU Chang-Yan. Study on Ultrafiltration Separation and Immunocompetence of Polysaccharides from Grifola frondosa[J]. FOOD SCIENCE, 2008, 29(9): 277-280.

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