食品科学 ›› 2008, Vol. 29 ›› Issue (9): 281-284.

• 工艺技术 • 上一篇    下一篇

骨肉酶解工艺条件研究

 许雄, 柳艳霞, 赵改名, 孙伟强, 张春晖, 蔡根旺   

  1. 河南农业大学食品科学技术学院; 双汇集团技术中心;
  • 出版日期:2008-09-15 发布日期:2011-12-08

Study on Enzymolysis Technology of Pork Femur Bone

XU  Xiong, LIU  Yan-Xia, ZHAO  Gai-Ming, SUN  Wei-Qiang, ZHANG  Chun-Hui, CAI  Gen-Wang   

  1. 1. College of Food Science and Technology,Henan Agricultural University,Zhengzhou 450002,China; 2. Technical Center of Shineway Group,Luohe 462000,China
  • Online:2008-09-15 Published:2011-12-08

摘要: 为了进行猪骨肉的综合利用,本实验以猪骨为原料,研究木瓜蛋白酶、中性蛋白酶和胰蛋白酶的水解效果以及最适蛋白酶的酶解温度、pH、酶用量、料液比和酶解时间对骨肉蛋白水解度和总氮回收率的影响。结果表明:胰蛋白酶为最佳骨肉水解酶,胰蛋白酶水解猪骨的最佳工艺参数为:酶解时间为5h、酶解温度60℃、pH8、酶用量5000U/g原料、料液比为1:5。

关键词: 骨肉, 胰蛋白酶, 酶解

Abstract:  Enzymolysis effects of papain, neutral protease and trypsin on pork femur bone were compared, and effects of enzyme concentration, substrate concentration, enzymatic hydrolysis temperature, time and pH on the degree of hydrolysis (DH) and total nitrogen recovery for the optimal protease were also discussed. The results showed that trypsin is the optimal protease to hydrolyze pork femur bone, and its best hydrolysis parameters are time 5 h, temperature 60 ℃, pH 8, enzyme concentration 5000 U/g raw material and substrate concentration 1:5.

Key words: pork femur bone, trypsin, enzymatic hydrolysis