食品科学 ›› 2009, Vol. 30 ›› Issue (13): 13-17.doi: 10.7506/spkx1002-6630-200913001

• 基础研究 •    下一篇

草鱼蛋白源抗氧化肽的分离及鉴定

任娇艳1,赵谋明1,崔春1,游丽君1,王海燕2   

  1. 1. 华南理工大学轻工与食品学院
    2. 上海烟草(集团)公司技术中心
  • 收稿日期:2008-11-07 修回日期:2009-01-16 出版日期:2009-07-01 发布日期:2010-12-29
  • 通讯作者: 赵谋明 E-mail:femmzhao@scut.edu.cn
  • 基金资助:

    国家“863”计划重点项目(2007AA100404);“十一五”国家科技支撑计划项目(2006BAD27B03);
    广东省科技计划项目(2006A25006001)

Isolation and Identification of Antioxidant Peptides from Hydrolyzed Grass Carp Protein

REN Jiao-yan1,ZHAO Mou-ming1,*,CUI Chun1,YOU Li-jun1,WANG Hai-yan2   

  1. 1. College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China;
    2. Technology Centre, Shanghai Tobacco (Group) Corporation, Shanghai 200082, China
  • Received:2008-11-07 Revised:2009-01-16 Online:2009-07-01 Published:2010-12-29
  • Contact: ZHAO Mou-ming E-mail:femmzhao@scut.edu.cn

摘要:

利用超滤、离子交换色谱、高效逆流色谱以及凝胶过滤色谱等系列分离纯化技术从草鱼蛋白酶解产物中分离纯化抗氧化活性肽,分离过程中发现相对分子质量1~3kD 的组分抗氧化活性最强,且碱性肽组分的抗氧化活性强于酸性或中性肽组分、疏水性肽组分的抗氧化活性强于亲水性肽组分。最终借助反相高效液相色谱在线连接的电喷雾质谱结合氨基酸分析鉴定出一种抗氧化肽,一级结构序列为Pro-Ser-Lys-Tyr-Glu-Pro-Phe-Val,相对分子质量为966.3D。

关键词: 草鱼, 抗氧化肽, 分离, 氨基酸序列, 电喷雾质谱

Abstract:

Through separation of grass carp protein hydrolysates (GCPHs) by ultrafiltration and consecutive chromatographic methods including ion exchange chromatography (IEC), high-speed counter-current chromatography (HSCCC), and gel filtration chromatography (GFC), a peptide fraction with the relative molecular mass ranging 873-3139 D was obtained, which had the strongest antioxidant activity (the lowest IC50 for scavenging superoxide anion radical) compared to other two peptide fractions having the relative molecular mass ranging 379-873 D and 222-379 D, respectively. Basic peptides from hydrolyzed grass carp protein demonstrated greater antioxidant activity than the acidic or the neutral ones, and hydrophobic peptides in the GCPHs presented higher activities than the hydrophilic ones. By means of online RP-HPLC/ESI-MS, the amino acid sequence of the peptide with the strongest antioxidant activity was confirmed as Pro-Ser-Lys-Tyr-Glu-Pro-Phe-Val, and its relative molecular mass was determined as 966.3 D.

Key words: grass carp, antioxidant peptides, isolation, amino acid sequence, electrospray ionization mass spectrometry

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