食品科学 ›› 2010, Vol. 31 ›› Issue (16): 106-110.doi: 10.7506/spkx1002-6630-201016022

• 工艺技术 • 上一篇    下一篇

响应曲面法优化北五味子总三萜的提取工艺

李 斌,李元甦,孟宪军* ,薛 雪,汪艳群   

  1. 沈阳农业大学食品学院
  • 收稿日期:2010-04-02 修回日期:2010-07-06 出版日期:2010-08-25 发布日期:2010-12-29
  • 通讯作者: 孟宪军 E-mail:mengxjsy@126.com
  • 基金资助:

    “十一五”国家科技支撑计划项目(2006BAI09B04)

Optimization of Extraction of Triterpenoids from Schisandra chinensis (Turcz.) Baill by Response Surface Methodology

LI Bin,LI Yuan-su,MENG Xian-jun*,XUE Xue,WANG Yan-qun   

  1. College of Food Science, Shenyang Agricultural University, Shenyang 110866, China
  • Received:2010-04-02 Revised:2010-07-06 Online:2010-08-25 Published:2010-12-29
  • Contact: MENG Xian-jun E-mail:mengxjsy@126.com

摘要:

在单因素试验基础上,利用响应曲面法中心组合设计,采用超声波辅助萃取法对北五味子总三萜提取工艺参数进行优化分析。选择频率、时间和乙醇体积分数作为优化因素,研究不同因素水平对北五味子总三萜得率的影响。通过响应面分析得到总三萜提取的最优条件:频率68Hz、提取时间48min、乙醇体积分数64%。在此条件下,北五味子总三萜的得率为(2.534 ± 0.244)%,与模型高度拟合。

关键词: 响应曲面法, 北五味子, 总三萜, 提取

Abstract:

On the basis of one-factor-at-a-time investigations, a 20-run central composite design (CCD) involving in 3 factors at 5 levels was used to provide experimental data for optimizing the utrasonic-assisted extraction of triterpenoids from Schisandra chinensis (Turcz.) Baill by response surface methodology (RSM). The optimum conditions for the utrasonic-assisted extraction of triterpenoids from Schisandra chinensis (Turcz.) Baill were determined to be: ultrasonic frequency 68 Hz, length of extraction 48 min and ethanol concentration 64%. Under the optimized conditions, the experimental yield of triterpenoids was (2.534 ± 0.244)%, which was in good agreement with its predicted counterpart.

Key words: response surface methodology, Schisandra chinensis, triterpenoids, extraction

中图分类号: