苄嘧磺隆人工抗原的合成与鉴定

食品科学 ›› 2012, Vol. 33 ›› Issue (19): 223-226.

苄嘧磺隆人工抗原的合成与鉴定

王磊1，睢珂1,2，王志伟1,2,3，潘家荣1,*，赵杰2，郑月明

1.中国计量学院生命科学学院 2.中国农业科学院农产品加工研究所 3.沈阳农业大学食品学院

收稿日期:2011-09-26 修回日期:2012-08-13 出版日期:2012-10-15 发布日期:2012-09-17
通讯作者: 王磊 E-mail:wangstone820801@sina.com
基金资助:
“十一五”国家科技支撑计划项目

Synthesis and Identification of Artificial Antigen for Bensulfuron

Wang Lei,

Received:2011-09-26 Revised:2012-08-13 Online:2012-10-15 Published:2012-09-17

摘要/Abstract

摘要： 以2-甲酸甲酯苄磺酰胺和琥珀酸酐为原料合成半抗原(hapten)，经薄层层析和1H-NMR鉴定后，采用混合酸酐法分别与牛血清蛋白(BSA)和卵清白蛋白(OVA)偶联获得免疫原(hapten-BSA)和包被原(hapten-OVA)，紫外光谱分析法计算得其偶联比分别为8:1和13:1，初步说明具有较好免疫原性。通过动物免疫实验获得了效价达1:32000的阳性血清，采用间接酶联免疫吸附检测(ELISA)法检测抗体的IC50和IC10值为0.5032mg/L和0.0024mg/L，表明人工抗原合成成功。

关键词: 苄嘧磺隆, 磺隆除草剂, 酶联免疫吸附检

Abstract: In this study, a hapten was synthesized from methyl 2-[(sulfamoyl)methyl] benzoate and succinic anhydride. The synthetic hapten was identified by thin layer chromatography and 1H-NMR spectroscopy. An immune antigen (hapten-BSA) and a coating antigen (hapten-OVA) were prepared through separate conjugation of the hapten with BSA and OVA by mixed anhydride method. The conjugation ratios of the immune antigen and coating antigen were calculated to be 8:1 and 13:1 based on UV spectroscopic analysis, respectively, suggesting their good immunogenicity. Positive sera with a titer of 1:32000 were obtained by animal immunization trials. The IC50 and IC10 of antibody were determined by indirect ELISA assay to be 0.5032 mg/L and 0.0024 mg/L, respectively. Therefore, the synthesis of artificial antigen is successful.

Key words: bensulfuron, sulfonylurea herbicide, ELISA

中图分类号:

TS207.3

王?磊1，睢?珂1,2，王志伟1,2,3，潘家荣1,*，赵?杰2，郑月明. 苄嘧磺隆人工抗原的合成与鉴定[J]. 食品科学, 2012, 33(19): 223-226.

Wang Lei. Synthesis and Identification of Artificial Antigen for Bensulfuron[J]. FOOD SCIENCE, 2012, 33(19): 223-226.

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