18S rDNA和ITS相结合鉴定一株盐生海芦笋内生真菌的研究

食品科学 ›› 2012, Vol. 33 ›› Issue (21): 173-176.

18S rDNA和ITS相结合鉴定一株盐生海芦笋内生真菌的研究

王惠，湛东锐，李连强，曹娜，赵育卉，刘天行，孟晓露，辛志宏*

南京农业大学食品科技学院

收稿日期:2012-03-19 修回日期:2012-09-14 出版日期:2012-11-15 发布日期:2012-11-09
通讯作者: 辛志宏 E-mail:xzhfood@163.com
基金资助:
国家自然科学基金面上项目;2011年国家大学生创新训练计划

Identification of a Halophilic Endophytic Fungus Isolated from Salicornia bigelovii

Received:2012-03-19 Revised:2012-09-14 Online:2012-11-15 Published:2012-11-09
Contact: zhihong xin E-mail:xzhfood@163.com

摘要/Abstract

摘要： 对分离自盐生海芦笋的一株代号为Salicorn 8的真菌进行鉴定，提取基因组DNA后分别PCR扩增18S rDNA和ITS区域，基因测序并进行同源性分析，构建ITS基因系统发育树，结合形态学分析，将该菌鉴定为木贼镰刀菌(Fusarium equiseti)。

关键词: 海芦笋, 鉴定, 18S rDNA基因, ITS基因, 系统发育分析

Abstract: A fungus coded Salicorn 8 was isolated from the halophyte Salicornia bigelovii. Its 18S rDNA and ITS region were amplified by PCR and then sequenced after extraction of genomic DNA. Homology analysis was conducted and ITS gene phylogenetic tree was constructed. Together with morphological analysis, the strain was identified as Fusarium equiseti.

Key words: Salicornia bigelovii, identification, 18S rDNA gene, ITS gene, phylogenetic analysis

中图分类号:

TS255.1

王?惠，湛东锐，李连强，曹?娜，赵育卉，刘天行，孟晓露，辛志宏*. 18S rDNA和ITS相结合鉴定一株盐生海芦笋内生真菌的研究[J]. 食品科学, 2012, 33(21): 173-176.

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