复合酶酶法提取荷叶黄酮类化合物的研究

摘要/Abstract

摘要： 研究复合酶解法提取荷叶黄酮类化合物的最佳工艺条件。参考料液比、pH值、加酶量、温度和时间5个因素对提取率的影响，在单因素试验基础上用正交试验优化提取条件。选择70%乙醇溶液为溶剂，料液比l:20(g/mL)，选取酶解pH值、加酶量、酶解温度和时间4个因素，对酶解法提取荷叶黄酮类化合物进行L16(44)正交试验，得到荷叶黄酮类化合物的最佳提取条件为pH3.5、时间3.0h、温度35℃、复合酶用量2800U/g纤维素酶+2000U/g果胶酶，荷叶黄酮提取率为3.74%。与水提法的L16(44)正交试验结果(提取率1.59%)相比较，复合酶解法提取荷叶黄酮类化合物的效果显著(P＜0.01)。

关键词: 黄酮类化合物, 荷叶, 复合酶解法, 提取效率

Abstract: This study aimed at determining the optimum hydrolysis conditions for the extraction of flavonoids from lotus leaves. Cellulase and pectinase were used in combination for one-step hydrolysis of lotus leaves and the solvent used was 70%. The effects of five extraction conditions including solid-to-solvent ratio, pH, enzyme dosage, temperature and hydrolysis time on extraction efficiency were investigated by one-factor-at-a-time method. The optimum solid-to-solvent ratio was found to be 1:20 g/mL. pH, enzyme dosage, temperature and hydrolysis time were optimized using a 16-run orthogonal array design to be 3.5, 3.0 h, 35 ℃, 2800 U/g cellulase + 2000 U/g pectinase, respectively. Under the optimized conditions, the extraction yield of flavonoids was 3.74% compared with 1.59% for aqueous extraction technique as optimized also using a 16-run orthogonal array design (P＜0.01). Hence, we concluded that enzymatic hydrolysis provides a high-yield method for the extraction of flavonoids from lotus leaves.

Key words: flavonoid, lotus leaf, enzymatic hydrolysis, extraction efficiency

中图分类号:

R248.2

张胜帮，叶?静，陈?聪. 复合酶酶法提取荷叶黄酮类化合物的研究[J]. 食品科学, 2012, 33(22): 149-153.

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