响应面优化草鱼鱼鳔酶溶胶原蛋白的提取工艺

食品科学 ›› 2012, Vol. 33 ›› Issue (22): 154-157.

响应面优化草鱼鱼鳔酶溶胶原蛋白的提取工艺

任国艳，詹永献，郭金英，梁旺春，刘志龙，史明科

河南科技大学食品与生物工程学院

收稿日期:2011-11-15 修回日期:2012-10-19 出版日期:2012-11-25 发布日期:2012-11-20
通讯作者: 任国艳 E-mail:guoyan.ren@yahoo.com.cn

Optimization of Enzymatic Extraction Process for Collagen from Grass Carp Bladders by Response Surface Methodology

Received:2011-11-15 Revised:2012-10-19 Online:2012-11-25 Published:2012-11-20
Contact: Guo-Yan REN E-mail:guoyan.ren@yahoo.com.cn

摘要/Abstract

摘要： 为提高草鱼下脚料鱼鳔中胶原蛋白的提取率，采用响应面法优化胃蛋白酶提取草鱼鱼鳔胶原蛋白。探讨加酶量、提取时间和液料比3个因素对提取率的影响；在单因素试验的基础上进行响应面优化试验。结果表明：胃蛋白酶提取草鱼鱼鳔胶原蛋白的最佳工艺条件为加酶量40mg/g、提取时间48h、液料比80:1(mL/g)，在此工艺条件下胶原蛋白实际提取率为17.82%，与模型预测值(18.04%)接近。方差分析显示，模型可靠，3个因素均对草鱼鱼鳔胶原蛋白的提取率影响显著。

关键词: 响应面优化, 胃蛋白酶, 草鱼鱼鳔, 胶原蛋白

Abstract: The pepsin-catalyzed hydrolysis of grass carp bladders was optimized by response surface methodology to improve the extraction yield of collagen. We explored the effects of hydrolysis parameters, enzyme loading, hydrolysis time and liquid-to-solid ratio on the extraction yield of collagen. Their optimal values for collagen extraction were found to be 40 mg/g, 48 h and 80:1 (mL/g), respectively. Under the optimized conditions, the actual extraction yield of collagen was 17.82%, which was close to the predicted value (18.04%). The results of analysis of variance showed that the fitted regression model was reliable, and each hydrolysis parameters had a significant effect on the extraction yield of collagen.

Key words: response surface methodology, pepsin, grass carp bladders, collagen

中图分类号:

任国艳，詹永献，郭金英，梁旺春，刘志龙，史明科. 响应面优化草鱼鱼鳔酶溶胶原蛋白的提取工艺[J]. 食品科学, 2012, 33(22): 154-157.

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