高效液相色谱法测定叶黄素顺、反异构体

食品科学 ›› 2012, Vol. 33 ›› Issue (22): 186-190.

高效液相色谱法测定叶黄素顺、反异构体

李大婧1,2,3，王闯1，徐爱琴2,3，刘春泉2,3,*

1.东北林业大学林学院 2.江苏省农业科学院农产品加工研究所 3.国家农业科技华东(江苏)创新中心农产品加工工程技术研究中心

收稿日期:2012-03-22 修回日期:2012-10-22 出版日期:2012-11-25 发布日期:2012-11-20
通讯作者: 刘春泉 E-mail:liuchunquan200@163.com
基金资助:
中央高校基本科研业务费专项;江苏省自然科学基金项目

Determination of Lutein Stereoisomers by HPLC

Received:2012-03-22 Revised:2012-10-22 Online:2012-11-25 Published:2012-11-20
Supported by:
;Natural Science Foundation of Jiangsu province

摘要/Abstract

摘要： 为了建立高效液相色谱法测定叶黄素顺、反异构体的方法, 通过比较C18和C30色谱柱对叶黄素异构化样品的分离效果，并采用高效液相色谱-二级管阵列检测器-大气压化学电离-质谱对叶黄素顺、反异构体进行定性定量分析。该方法C30色谱柱为固定相，以甲醇-水(98:2，V/V)为流动相，在40～200ng范围内峰面积和进样量呈良好的线性关系，且具有较高的准确度和良好的重现性。该方法经济可靠，可用于叶黄素顺、反异构体的检测。

关键词: 叶黄素, 顺、反异构体, 高效液相色谱法

Abstract: An HPLC-DAD-atmospheric pressure chemical ionization (APCI)-MS method was proposed to quantify and qualify lutein stereoisomers. A C30 column was found to be more effective in separating lutein stereoisomers than a C18 column. The mobile phase was composed of methanol and deionized water (98:2, V/V). A good relationship between peak area and injection amount was found in the range of 40 to 200 ng. The proposed method was accurate, reproducible, economical and suitable for the determination of lutein stereoisomers.

Key words: lutein, stereoisomers, high performance liquid chromatography (HPLC)

中图分类号:

TS201.2

李大婧1,2,3，王?闯1，徐爱琴2,3，刘春泉2,3,*. 高效液相色谱法测定叶黄素顺、反异构体[J]. 食品科学, 2012, 33(22): 186-190.

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