明日叶水溶性总黄酮提纯工艺

食品科学 ›› 2012, Vol. 33 ›› Issue (22): 27-32.

明日叶水溶性总黄酮提纯工艺

郭晓青1，吴金鸿2,*，周焱富3，王正武2，杨科峰4

1.贵阳护理职业学院 2.上海交通大学农业与生物学院，陆伯勋食品安全研究中心 3.福州大学生物科学与工程学院 4.上海交通大学医学院

收稿日期:2012-07-13 修回日期:2012-10-24 出版日期:2012-11-25 发布日期:2012-11-20
通讯作者: 吴金鸿 E-mail:wjh97@126.com
基金资助:
冰提丝胶肽结构特性及抗冻作用机制的研究;基于SAXS的B型芭蕉芋淀粉晶体结构的模型构建及其酶解机理;低碳农业产品芭蕉芋的高值化产品中试和示范;药食同源新蔬菜品种明日叶深加工关键技术与示范;药食兼用明日叶泡腾片固体饮料抗糖尿病作用效果与机理研究

Extraction and Purification of Water-Soluble Flavonoids from Angelica keiskei Koidzumi Leaves

Received:2012-07-13 Revised:2012-10-24 Online:2012-11-25 Published:2012-11-20

摘要/Abstract

摘要： 比较水浴加热、直接加热煮沸、微波辅助浸提3种水浸提取方法，确定明日叶叶总黄酮提取率最高的水浸提工艺条件，得到粗提液，并进一步考察大孔树脂类型、粗提液上样量、乙醇洗脱液pH值、乙醇体积分数、洗脱液用量等条件对水溶性总黄酮提纯结果的影响。结果表明：水浴加热浸提的提取率最高，其最佳浸提条件为水浴温度95℃，加热时间10min。HP-20大孔树脂对明日叶叶中总黄酮具有较好吸附与解吸性能，最佳提纯工艺条件为质量浓度为2.445mg/mL的粗提液上样量为40mL，洗脱液乙醇体积分数70%、pH2、洗脱用量为8BV。提纯产物总黄酮含量比明日叶叶干粉的含量提高了约6.8倍。

关键词: 明日叶, 水溶性总黄酮, 大孔树脂, 水浸提, 提纯

Abstract: In the present study, three aqueous extraction techniques, water bath extraction, direct heating extraction and microwave-assisted extraction were compared to determine the most suitable one for maximum extraction yield of water-soluble flavonoids from Angelica keiskei Koidzumi leaves. The water bath extraction method provided maximum extraction of water-soluble flavonoids and the optimal extraction conditions were heating at 95 ℃ for 10 min. Further investigations were carried to explore the purification of the resulting crude extract under various conditions of macroporous resin type, sample loading amount, and elution solvent (ethanol) pH, ethanol concentration and elution volume. HP-20 type resin was found to have higher adsorption and desorption capacities for flavonoids and the optimal conditions for purifying water-soluble flavonoids from Angelica keiskei Koidzumi leaves using the resin were determined as follows: 40 mL of crude extract sample at 2.445 mg/mL were loaded onto HP-20 type resin and then desorbed with 8 BV of 70% acidified ethanol (pH 2), resulting in a purification factor of approximately 6.8.

Key words: Angelica keiskei Koidzumi, water-soluble flavonoids, macroporous resin, water extraction, purification

中图分类号:

R284.2

郭晓青1，吴金鸿2,*，周焱富3，王正武2，杨科峰4. 明日叶水溶性总黄酮提纯工艺[J]. 食品科学, 2012, 33(22): 27-32.

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