食品科学 ›› 2012, Vol. 33 ›› Issue (22): 22-26.

• 工艺技术 • 上一篇    下一篇

响应面法优化高溶解性花生蛋白提取工艺

周婷婷1,郑 杨2,吕 茜1,张玉玉1,宋 弋1,李全宏1,*   

  1. 1.中国农业大学食品科学与营养工程学院,国家果蔬加工工程技术研究中心 2.北京工商大学食品学院
  • 收稿日期:2011-09-13 修回日期:2012-10-19 出版日期:2012-11-25 发布日期:2012-11-20
  • 通讯作者: 李全宏 E-mail:liquanhong66@163.com
  • 基金资助:

    微波作用下塑料包装材料化学物迁移动力学研究

Optimization of Extraction Conditions for Improving Solubility of Peanut Proteins by Response Surface Methodology

,Quan-hong LI   

  • Received:2011-09-13 Revised:2012-10-19 Online:2012-11-25 Published:2012-11-20
  • Contact: Quan-hong LI E-mail:liquanhong66@163.com

摘要: 为开发脱脂花生粕中的蛋白质,研究提取温度、提取pH值、液料比对两种花生蛋白组分——花生球蛋白和伴花生球蛋白溶解性的影响,并以响应面法设计优化提取条件,结果表明:提取温度62.93℃、提取pH9.04、液料比15.11:1(mL/g)时,两种花生蛋白溶解性最好。验证实验得到花生球蛋白的NSI为54.82%,伴花生球蛋白的NSI为74.1%,与模型预测值接近。采用响应面法对花生蛋白组分提取条件进行优化合理可行。

关键词: 花生球蛋白, 伴花生球蛋白, 溶解性, 响应面分析

Abstract: This work reports the use of response surface methodology to optimize conditions for the extraction of arachin and conarachin from defatted soybean meal. Defatted soybean meal was suspended in 0.03 mol/L Tris-HCl buffer at a constant temperature and stirred for 1 h. As a result, arachin and conarachin were extracted into the supernatant. Temperature, pH and solvent/solid ratio were tested for their effects on the solubility of arachin and conarachin. The best solubility of arachin and conarachin was observed when the extraction conditions were 62.93 ℃, pH 9.04 and 15.11:1 (mL/g). In addition, the experimental NSI values of arachin and conarachin were 54.82% and 74.1%, respectively, which were close to the predictive values. Accordingly, response surface methodology can be a reasonable and feasible approach to optimizing conditions for the extraction of peanut proteins.

Key words: arachin, conarachin, solubility, response surface methodology

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