超声波-表面活性剂协同提取竹叶总黄酮的工艺

食品科学 ›› 2012, Vol. 33 ›› Issue (22): 76-80.

超声波-表面活性剂协同提取竹叶总黄酮的工艺

马祥，晏日安*

暨南大学食品科学与工程系

收稿日期:2011-09-22 修回日期:2012-09-05 出版日期:2012-11-25 发布日期:2012-11-20
通讯作者: 晏日安 E-mail:yanrian813@163.com
基金资助:
广东高校科技成果转化项目;广东省重大科技专项

Ultrasound-assisted Surfactant Extraction of Total Flavonoids from Bamboo Leaves

Received:2011-09-22 Revised:2012-09-05 Online:2012-11-25 Published:2012-11-20

摘要/Abstract

摘要： 研究超声波-表面活性剂协同提取竹叶总黄酮的工艺。在单因素试验的基础上，采用响应面法研究超声功率、表面活性剂浓度和液固比对竹叶总黄酮得率的影响。结果表明，在超声频率40kHz和常温下，超声波-表面活性剂协同提取竹叶总黄酮的最佳工艺为超声功率420W、十二烷基硫酸钠物质的量浓度25.20×10-3mol/L、液固比23:1(mL/g，表面活性剂溶液:竹叶粉末)，此时竹叶总黄酮得率达2.30%。

关键词: 超声波, 表面活性剂, 竹叶, 总黄酮

Abstract: The ultrasonic-assisted surfactant extraction of total flavonoids from bamboo leaves was optimized by one-factor-at-a-time method and response surface methodology. The extraction yield of total flavonoids was investigated with respect to ultrasound power, surfactant type, surfactant critical micelle (CMC) concentration and solvent-to-solid ratio. The results showed that the optimal conditions for the extraction of total flavonoids at 40 kHz ultrasonic power and ambient temperature were ultrasound power of 420 W, SDS as the extraction surface at a CMC of 25.20×10-3 mol/L, and a solvent-to-solid ratio of 23:1 (mL/g) (ratio of SDS solution to bamboo leaves). Under these conditions, the yield of total flavonoids was 2.30%.

Key words: ultrasound, surfactant, bamboo, total flavonoids

中图分类号:

TS207.3

马?祥，晏日安*. 超声波-表面活性剂协同提取竹叶总黄酮的工艺[J]. 食品科学, 2012, 33(22): 76-80.

参考文献

参考文献
[1] 朱石麟,马乃训,傅懋毅.中国竹类植物图志[M].北京:中国林业出版社,1994.
[2] 周惠燕,章辉,李士敏.竹叶化学成分研究Ⅰ[J].中国中药杂志,2005,30(24):1933-1934.
[3] 周惠燕,李士敏.竹叶化学成分研究[J].中国药学杂志,2006,41(9):662-663.
[4] 吴三林,李书华,弓加文.竹叶活性成分的研究进展[J].乐山师范学院学报,2005,20(5):53-54.
[5] 黄文,王益.竹叶的化学成份及应用进展[J].中国林副特产,2002,62(3):65-66.
[6] LU Y, FOO LY. Antioxidant activities of polyphenols from sage (Salviaofficinalis) [J]. Food Chemistry, 2001, 75(2):197–202.
[7] HARBONE JB, WILLIAMAS CA. Advances in flavonoid research since 1992[J]. Phytochemistry, 2000, 55(6):481–504.
[8] HAVSTEEN BH. The biochemistry and medical significance of the flavonoids[J]. Pharmacology & Therapeutics, 2002, 96(2-3):67–202.
[9] KIM MH. Flavonoids inhibit VEGF/bFGF-induced angiogenesis in vitro by inhibiting the matrix-degrading proteases [J]. Journal of Cell Biochemistry, 2003, 89(3):529–538.
[10] FOTSIS T, PEPPER MS, ATKAS E, et al. Flavonoids, dietary-derived inhibitors of cell proliferation, and in vitro angiogenesis[J]. Cancer Resarch, 1997, 57:2916–2921.
[11] BAGLI E, STEFANIOTOU M, MORBIDELLI L, et al. Luteolin inhibits vascular endothelial growth factor-induced angiogenesis; inhibition of endothelial cell survival and proliferation by targeting phosphatidylinositol 3`-kinase activity[J]. Cancer Research, 2004, 64:7936–7946.
[12] 孙宝国,何坚.香料化学与工艺学[M].北京:化学工业出版社,2004.
[13] SCHRADER J, ETSCHMANN M M W, SELL D, et al. Applied biocatalysis for the synthesis of natural flavour compounds-current in-dustrial processes and future prospects [J].Biotechnology Letters, 2004, 26(6):463-472.
[14] 张晓玲,惠芸华,杨桥微.生物发酵产大豆异黄酮苷元的连续超声波提取工艺及其定量分析研究[J].食品科学,2011,32(5):239-243.
[15] 李敏,杨建华,李渊,胡君萍.酒花黄酮提取工艺和含量测定[J].食品科学,2011,32(6):16-19.
[16] 孔涛,范杰平,胡小芳等.响应面法优化超声辅助提取车前草中的熊果酸[J].食品科学,2011,32(6):80-84.
[17] 史礼貌,解成喜.新疆大蓟总黄酮的超声提取及抗氧化性研究[J].食品科学,2011,32(6):120-123.
[18] 龚盛昭,程江,杨卓如.表面活性剂协同提取芦丁研究[J].林产化学与工业, 2004, 24 (S1): 93-96.
[19] 胡楠,方波,陈晶晶等.表面活性剂强化微波提取花生壳黄酮的工艺研究[J].食品科技,2009,34(3):200-203.
[20] 杨铭,余林.表面活性剂-微波协同提取灯心总草黄酮的研究[J].中国食品添加剂, 2009, (6): 95-99.
[21] 陈伟,陈惠,刘晓燕等.竹叶黄酮的提取和测定研究进展[J].中国食品添加剂,2007,73(4):73-76.
[22] 杨转萍,罗仓学,李祥.超声波-表面活性剂协同萃取黄姜中薯蓣皂苷的工艺研究[J].食品科学,2010,31(22):46-49.
[23] AMINI M,YOUNESI H. Application of response surface methodology for optimization of lead biosorption in an aqueous solution by Aspergil-lus niger[J].Journal of Hazardous Materials,2008,154(1-3):694-702.
[24] WU T,ANNIE B S W,GU L. Simultaneous determination of sixiso flavonoids in commercial Radix Astragali by HPLC-UV[J]. Fitoterapia, 2005, 76(2):157-165.
[25] WANI A, KAUR D, AHMED I. Extraction optimization of watermelon seed protein using response surface methodology [J].LWT-Food Science and Technology, 2008, 41(8):1514-1520.
[26] 吴华勇,黄赣辉,顾振宇等.响应曲面法优化竹叶总黄酮的提取工艺研究[J].食品科学,2008,29(11):196-200.
[27] 陈海光,黄敏,于立梅.超声波辅助提取青皮竹叶黄酮工艺的研究[J].食品工业科技,2010,31(9):245-247.
[28] 张珊珊,赵晓红.不同提取方法对北京市早园竹叶黄酮提取效率比较[J].食品科技,2008,3:167-170.
[29] 宋春芳,翟新,于震等.真空微波预辐射辅助提取竹叶黄酮工艺的优化[J]. 西北农林科技大学学报（自然科学版）,2010,38(10):192-198.
[30] TANG H G, LI Y, XIANG J L, et al. Study on purification of flavonoids from Leaves of Dendrocalamuslatiflorus by ultrafiltration[J].Food Science,2008,19(5):177-180.