关键词: 三氯氧磷, 化学改性, 玉米抗性淀粉, 急性毒理学实验, 亚慢性毒理学实验

Abstract: In order to evaluate the safety of long-term consumption of chemically modified maize resistant starch prepared with POCl3, acute toxicity test was used to determine its safety dosage. For this, the modified starch was given to mice by gavage at four different doses, and median lethal dose (LD50) and 95% fiducial limits (95% FL) were calculated by Bliss method. According to the results of acute toxicology test, 4 gradients 90% LD50, 75% LD50, 50% LD50, and 25% LD50 were designed for a 90-day subchronic toxicity test. The results showed that the LD50 of the modified starch was 33.9 g/kg and the 95% FL was 23.7–48.3 g/kg, indicating that this material was non-toxic. Moreover, its acceptable daily intake for a normal person (65 kg) should be less than 16526.25 mg, which means that the recommended intake (RNI) is no more than 16.526 g/d.

Key words: POCl3, chemical modification, maize resistant starch, acute toxicity test, subchronic toxicity test