食品科学 ›› 2013, Vol. 34 ›› Issue (1): 189-193.

• 生物工程 • 上一篇    下一篇

章鱼肠道产蛋白酶菌的筛选、产酶条件及酶学性质

任 佩1,金玉兰2,朴美子1,*   

  1. 1.青岛农业大学食品科学与工程学院 2.青岛农业大学化学与药学院
  • 收稿日期:2011-11-17 修回日期:2012-12-20 出版日期:2013-01-15 发布日期:2013-01-07
  • 通讯作者: 朴美子 E-mail:piaomeizi2009@126.com
  • 基金资助:

    山东省自然科学基金项目

Screening of Protease-Producing Bacterium from Octopus vulgaris Intestine, Fermentation Conditions and Enzymatic Properties

,Jin Yu Lan,mei-zi PIAO   

  • Received:2011-11-17 Revised:2012-12-20 Online:2013-01-15 Published:2013-01-07
  • Contact: mei-zi PIAO E-mail:piaomeizi2009@126.com

摘要: 采用检测水解透明圈和测定蛋白酶活力的方法,从章鱼肠道中分离筛选出一株高产蛋白酶的菌株QDV-3,并对其产酶条件及酶学性质进行研究。结果表明:QDV-3菌株在以果糖为碳源,蛋白胨为氮源,培养基初始pH8.0,培养温度30℃的条件下振荡培养3.5d时,所产蛋白酶活力最高,Mn2+和Ba2+对菌株产蛋白酶有促进作用。所产蛋白酶在SDS-PAGE电泳上显示至少有5种蛋白酶,分子质量范围为32.4~124.2kD,蛋白酶的最适作用温度为50~60℃,最适作用pH值为9~11,在50℃条件下保温1h,剩余酶活力为95%,热稳定性较好。

关键词: 产蛋白酶菌, 蛋白酶, 酶学性质

Abstract: A protease-producing bacterial strain was isolated and screened from the intestine of Octopus vulgaris by detecting hydrolysis circle of protease and its activity and identified as QDV-3. The optimal fermentation conditions for protease production and its enzymatic properties were also investigated. The highest protease production was achieved when strain QDV-3 was cultured at 30 ℃ for 3.5 d under shaking conditions in a medium at pH 8.0 with fructose as the carbon source and peptone as the nitrogen source. Meanwhile, protease production was improved in the presence of Mn2+ and Ba2+. At least five proteases were identified by SDS-PAGE, with molecular weights between 32.4 kD and 124.2 kD. The optimal reaction temperature and pH for the fermentation supernatant were 50—60 ℃ and 9—11, respectively. The fermentation supernatant exhibited high thermal stability, and 95% of its initial activity was retained after 1 h incubation at 50 ℃.

Key words: protease-producing bacterial strain, protease, enzymatic properties