食品科学 ›› 2013, Vol. 34 ›› Issue (4): 114-117.

• 工艺技术 • 上一篇    下一篇

响应面法优化桑黄发酵液总多糖的提取工艺

梁大勇1,赵晨2,黄芳2,宋爱荣1   

  1. 1. 青岛农业大学山东省应用真菌重点实验室
    2. 青岛农业大学
  • 收稿日期:2012-02-06 修回日期:2013-01-16 出版日期:2013-02-25 发布日期:2013-01-29
  • 通讯作者: 宋爱荣 E-mail:airongsong@126.com
  • 基金资助:

    药用真菌提取物抗禽流感病毒的研究

Optimization of Extraction Technique of Polysaccharides from Phellinus igniarius Fermentation Liquor by Response Surface Methodology

  • Received:2012-02-06 Revised:2013-01-16 Online:2013-02-25 Published:2013-01-29

摘要:

为确定桑黄发酵液多糖提取的最佳工艺条件,以桑黄多糖的含量为考察指标,在单因素试验的基础上,采用响应面法对主要提取工艺参数进行了优化,并得到了回归模型。研究结果表明:桑黄发酵液多糖提取的最优工艺条件参数为提取pH12,提取温度96℃,提取时间1.6h。在此条件下,桑黄多糖含量达到11.67mg/ml。实验证明模型拟合程度良好,误差较小,模型的选择合适。

Abstract:

Optimal processing parameters for extraction of Phellinus igniarius polysaccharides in fermentation liquor with the content of polysaccharides as index were determined. On the basis of single factor experiments, the method of response surface method(RSM) for optimal parameters was adopted. Finally,The results of the study shows the optimal extraction process was obtained as follows: extraction pH12, extraction temperature 96℃, extraction time 1.6h. Under these conditions,the content of Phellinus igniarius polysaccharides reached 11.67mg/ml. The model turned out to be with good fitting degree and good feasibility.

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