高效液相色谱法对葡萄酒中纽甜的快速筛查与测定

食品科学 ›› 2013, Vol. 34 ›› Issue (4): 154-156.

高效液相色谱法对葡萄酒中纽甜的快速筛查与测定

王齐¹,朱伟伟²,杨俊¹

1. 昆明理工大学
2. 河池学院

收稿日期:2012-02-07 修回日期:2013-01-16 出版日期:2013-02-25 发布日期:2013-01-29
通讯作者: 王齐 E-mail:kmuac@163.com
基金资助:
云南省科技平台项目

The fast qualitative analysis and accurate quantitative analysis of neotame in wine by high performance liquid chromatography method

Received:2012-02-07 Revised:2013-01-16 Online:2013-02-25 Published:2013-01-29

摘要/Abstract

摘要：

建立一种高效液相色谱法快速定性筛查与准确定量检测葡萄酒中人工合成甜味剂纽甜的方法。采用Waters Nova-Pak C18 反相色谱柱，在218nm波长处，以离子对试剂缓冲液：乙腈=60:40(V/V)为流动相，对样品进行筛查，纽甜出峰时间约在13.4min；疑似阳性样品在梯度洗脱条件下进行定量检测，纽甜出峰时间约在15.9min，检出限为0.37μg/mL，平均回收率在99%～101%之间，相对标准偏差均小于1.0%(n=5)。所建立的方法简便、准确、实用，在批量样品检测中可节省约50%的分析时间.

Abstract:

A high performance liquid chromatography (HPLC) method for the fast qualitative analysis and accurate quantitative analysis of the artificial sweetener neotame in wines was developed. Neotame was fast qualitative analysis on a Waters Nova-Pak C18 column with an ultraviolet detector at 218 nm using acetonitrile: ion-pair reagent=40:60 (V/V) as mobile phase, and the retention time of neotame was about 13.4 min. The contents of neotame in the suspected samples were determined under gradient elution condition, and the retention time of neotame was about 15.9 min. The detection limit of the developed method was 0.37 μg/mL, and the mean spike recoveries for neotame in a blank wine sample were between 99%～101% , with a relative standard deviation below 1.0% (n=5). The method proved to be simple, accurate and practical, and it can save 50% time in analysis of large quantities of samples.

中图分类号:

TS207.3

王齐朱伟伟杨俊. 高效液相色谱法对葡萄酒中纽甜的快速筛查与测定[J]. 食品科学, 2013, 34(4): 154-156.

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