Abstract: Studies were made on extracting total flavonoids from Artemisia selengensis Turcz in this paper. Based on singer factor test, response surface method was used to study effects of extraction temperature, ethanol concentration, solid-liquid ratio and extraction time on extraction rate of total flavonoids from Artemisia selengensis Turcz, and a second order quadratic equation for total flavonoids extraction was finally obtained. The results showed that the model was of great difference, and total flavonoids from Artemisia selengensis Turcz was analyzed and predicted by the model. factors influencing the extraction of total flavonoids from the Artemisia selengensis Turcz by the water bath method were in the order as follows: extraction time＞ethanol concentration＞solid/liquid ratio(g/mL)＞extraction temperature. the optimum extraction conditions of total flavonoids from Artemisia selengensis Turcz were ethanol concentration 70%, extraction temperature 80℃, solid/liquid ratio(g/mL) 1:100, extraction time 180 minutes, the extraction rate of total flavonoids from Artemisia selengensis Turcz was 6.58% under the optimal conditions.