关键词: 二色补血草, 多糖, 乙酰化, 响应面, 抗氧化性

Abstract:

In this study, response surface methodology was employed to optimize the acetylation conditions of polysaccharides
from Limonium bicolor Kunze. The degree of substitution (Ds) of acetylated polysaccharides was investigated as a
function of reaction time, molar ratio of acetic anhydride to polysaccharides, and reaction temperature. The optimal conditions
for acetylating polysaccharides from Limonium bicolor Kunze were found to be reaction at 65 ℃ for 2.6 h with an
acetic anhydride-to-polysaccharide ratio of 3.3:1. The maximum DS of 0.409 was obtained under the optimized reaction
conditions. Antioxidant assays demonstrated that compared with Limonium bicolor Kunze polysaccharides, the antioxidant
capacity of acetylated derivatives was significantly improved.

Key words: Limonium bicolor Kunze, polysaccharides, acetylation, response surface methodology, antioxidant capacity