食品科学

• 基础研究 • 上一篇    下一篇

大果榕果实乙醇提取物抗氧化活性及对α-葡萄糖苷酶和乙酰胆碱酯酶抑制活性

谭 琳,郑晓燕,王甲水,马伏宁,康由发,臧小平,马蔚红   

  1. 1.中国热带农业科学院海口实验站,海南省香蕉遗传改良重点实验室,海南 海口 570102;
    2.海南大学城西校区管委会,海南 海口 571101
  • 出版日期:2016-07-15 发布日期:2016-07-26
  • 基金资助:

    中国热带农业科学院海口实验站科研启动项目(HKZKY140204);农业部财政项目

Antioxidant Activity and Inhibitory Activity of Ethanol Extract from Ficus auriculata Fruits against α-Glucosidase and Acetylcholine Esterase

TAN Lin, ZHENG Xiaoyan, WANG Jiashui, MA Funing, KANG Youfa, ZANG Xiaoping, MA Weihong   

  1. 1. Hainan Key Laboratory of Banana Genetic Improvement, Haikou Experimental Station, Chinese Academy of Tropical Agricultural
    Sciences, Haikou 570102, China; 2. Management Committee of Chengxi Campus, Hainan University, Haikou 571101, China
  • Online:2016-07-15 Published:2016-07-26

摘要:

研究大果榕果实的多酚含量及其抗氧化活性、α-葡萄糖苷酶和乙酰胆碱酯酶抑制活性,为其进一步开发利用奠定基础。以采自海南保亭七仙岭的大果榕果实为实验材料,用体积分数65%的乙醇提取大果榕果实多酚,采用Folin-酚法测定总酚含量。对获得的大果榕果实多酚进行1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基、2,2’-联氮双(3-乙基苯并噻唑啉-6-磺酸)二铵盐(2,2’-azinobis-(3-ethylbenzthiazoline-6-sulphonate),ABTS)自由基、羟自由基(•OH)清除率以及α-葡萄糖苷酶抑制活性和乙酰胆碱酯酶抑制活性测定。结果表明:大果榕果实多酚含量较高,达1.92 mg/g(以干质量计),其对DPPH自由基、ABTS+•和•OH均具有显著的清除活性,且呈质量浓度依赖性,IC50值分别为141.25、91.20、45.71 μg/mL;大果榕果实多酚对α-葡萄糖苷酶抑制活性强于阳性对照阿卡波糖,且呈现明显的质量浓度依赖性,IC50值为102.33 μg/mL;同时大果榕果实多酚对乙酰胆碱酯酶也具有一定的抑制活性,IC50值为 4.9 mg/mL。研究结果表明大果榕果实多酚具有良好的抗氧化性、α-葡萄糖苷酶抑制活性和一定的乙酰胆碱酯酶抑制活性。

关键词: 大果榕, 果实多酚, 抗氧化活性, 自由基清除活性, &alpha, -葡萄糖苷酶抑制活性, 乙酰胆碱酯酶抑制活性

Abstract:

Total phenolics were extracted from Ficus auriculata fruits using 65% ethanol and quantified by the Folin phenol
method. The antioxidant activity of the ethanol extract was assessed by DPPH, ABTS and hydroxyl radical scavenging
assays and its inhibitory activity against alpha-glucosidase and acetylcholine esterase was also evaluated. Results showed
that the fruit extract contained high levels of total phenolics (1.92 mg/g DW) and could scavenge DPPH, ABTS and hydroxyl
radicals effectively in a concentration dependent manner with IC50 of 141.25, 91.20 and 45.71 μg/mL, respectively. It also
exhibited strong inhibitory activity against α-glucosidase in a concentration dependent manner with IC50 of 102.33 μg/mL.
Similarly, it showed inhibitory activity against acetylcholine esterase with IC50 of 4.9 mg/mL. Therefore, the polyphenolrich
extract from Ficus auriculata fruits has strong antioxidant potential and inhibitory activity against α-glucosidase and
acetylcholine esterase.

Key words: Ficus auriculata, fruit polyphenols, antioxidant activity, radical scavenging activity, α-glucosidase inhibitory activity, acetylcholine esterase inhibitory activity

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