壶瓶枣多糖的纯化及结构初步分析

doi:10.7506/spkx1002-6630-201603007

食品科学

壶瓶枣多糖的纯化及结构初步分析

张耀雷1，黄立新1,*，张彩虹1,2，谢普军1，张琼1，丁莎莎1

1.中国林业科学研究院林产化学工业研究所，生物质化学利用国家工程实验室，国家林业局林产化学工程重点开放性实验室，
江苏省生物质能源与材料重点实验室，江苏南京 210042；2.中国林业科学研究院林业新技术研究所，北京 100091

出版日期:2016-02-15 发布日期:2016-02-26

Purification and Structural Analysis of Polysaccharides from Fruits of Zizyphus jujube Mill. cv. Hupingzao

ZHANG Yaolei1, HUANG Lixin1,*, ZHANG Caihong1,2, XIE Pujun1, ZHANG Qiong1, DING Shasha1

1. Jiangsu Province Key Laboratory of Biomass Energy and Material, Key and Open Laboratory of Forest Chemical Engineering, State Forestry Administration, National Engineering Laboratory for Biomass Chemical Utilization, Institute of Chemical Industry of Forest Products, Chinese Academy of Forestry, Nanjing 210042, China; 2. Research Institute of Forestry New Technology,Chinese Academy of Forestry, Beijing 100091, China

Online:2016-02-15 Published:2016-02-26

摘要/Abstract

摘要：

采用Sepharose CL-6B凝胶柱纯化壶瓶枣多糖（polysaccharides from Zizyphus jujube Mill. cv. Hupingzao，简称ZJP）ZJP-2和ZJP-5组分，并对纯化后多糖的结构进行分析。结果表明：经纯化后得到ZJP-2b和ZJP-5a两种组分均一的壶瓶枣活性多糖，分子质量分别为89.21、61.60 kD，均具备多糖的特征吸收峰，且均以β-构型的吡喃糖为主；ZJP-2b中单糖组成主要为鼠李糖、阿拉伯糖、甘露糖、葡萄糖和半乳糖，其物质的量比为32.4∶9.5∶9.4∶14.7∶9.7，而ZJP-5a中单糖组成主要为鼠李糖、阿拉伯糖、木糖、甘露糖和半乳糖，其物质的量比为20.2∶42.9∶2.2∶7.5∶14.5；当质量浓度为3.5 mg/mL时，ZJP-2b和ZJP-5a的羟自由基清除率分别为30.51%和57.22%。

关键词: 壶瓶枣, 活性多糖, 纯化, 结构

Abstract:

The polysaccharides (ZJP-2 and ZJP-5) from the fruits of Zizyphus jujube Mill. cv. Hupingzao were purified by
Sepharose CL-6B column chromatography, and the structures of the purified active polysaccharides were studied in this
investigation. The purified polysaccharides (ZJP-2b and ZJP-5a) were homogeneous, and their molecular weights were
89.21 and 61.60 kD, respectively. IR spectral analysis showed that ZJP-2b and ZJP-5a had the characteristic absorption
peaks of polysaccharide, and the main structure was β-pyranose for both polysaccharides. The polysaccharide ZJP-2b was
composed of rhamnose, arabinose, mannose, glucose and galactose with a molar ratio of 32.4:9.5:9.4:14.7:9.7, and ZJP-5a
was composed by rhamnose, arabinose, xylose, mannose and galactose with a molar ratio of 20.2:42.9:2.2:7.5:14.5. At the
concentration of 3.5 mg/mL, hydroxyl radical scavenging rates of ZJP-2b and ZJP-5a were 30.51% and 57.22%, respectively.

Key words: Zizyphus jujube Mill. cv. Hupingzao, active polysaccharides, purification, structure

中图分类号:

TQ929.2

张耀雷，黄立新，张彩虹，谢普军，张琼，丁莎莎. 壶瓶枣多糖的纯化及结构初步分析[J]. 食品科学, doi: 10.7506/spkx1002-6630-201603007.

ZHANG Yaolei, HUANG Lixin, ZHANG Caihong, XIE Pujun, ZHANG Qiong, DING Shasha. Purification and Structural Analysis of Polysaccharides from Fruits of Zizyphus jujube Mill. cv. Hupingzao[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201603007.

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壶瓶枣多糖的纯化及结构初步分析

Purification and Structural Analysis of Polysaccharides from Fruits of Zizyphus jujube Mill. cv. Hupingzao

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