氧化还原电位调控对钝齿棒杆菌代谢通量分布的影响

doi:10.7506/spkx1002-6630-201609031

食品科学

氧化还原电位调控对钝齿棒杆菌代谢通量分布的影响

陈小举1,2，操新民2，姜绍通2，李兴江2,*

1.巢湖学院化学与材料工程学院，安徽巢湖 238000；2.合肥工业大学食品科学与工程学院，安徽合肥 230009

出版日期:2016-05-15 发布日期:2016-05-18

Effect of Redox Potential Regulation on Metabolic Flux Distribution of Corynebacterium crenatum

CHEN Xiaoju1,2, CAO Xinmin2, JIANG Shaotong2, LI Xingjiang2,*

1. College of Chemistry and Material Engineering, Chaohu University, Chaohu 238000, China;
2. College of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China

Online:2016-05-15 Published:2016-05-18

摘要/Abstract

摘要：

研究不同氧化还原电位对钝齿棒杆菌厌氧发酵特性的影响，并对菌株的代谢通量分布特征进行了分析。结果表明，氧化还原电位由－56 mV降为－400 mV时，发酵液中琥珀酸质量浓度由14 g/L上升为20.2 g/L，乳酸质量浓度由44.9 g/L下降为35.2 g/L。代谢通量分析结果表明，降低氧化还原电位对6-磷酸葡萄糖与磷酸烯醇式丙酮酸节点处的代谢流分布影响显著。氧化还原电位为－400 mV时，胞内戊糖磷酸途径（pentose phosphate pathway，HMP）代谢通量与－56 mV相比增加了1.74 倍，由磷酸烯醇式丙酮酸流向C4途径的代谢通量与－56 mV相比增加了78%，琥珀酸通量由31.73 mmol/（L·g·h）增加到56.53 mmol/（L·g·h），乳酸代谢通量由159.73 mmol/（L·g·h）下降为133.50 mmol/（L·g·h）。研究结果表明6-磷酸葡萄糖与磷酸烯醇式丙酮酸节点是影响钝齿棒杆菌厌氧发酵产琥珀酸的关键节点，为后期通过菌种改造以调节乳酸和琥珀酸的生成比、实现乳酸与琥珀酸联产奠定了基础。

关键词: 钝齿棒杆菌, 氧化还原电位, 代谢通量分析, 厌氧发酵

Abstract:

For the co-production of lactic acid and succinic acid, the effect of redox potential regulation on the fermentation
process of Corynebacterium crenatum was studied and the metabolic flux distribution was also analyzed. When the redox
potential level was changed from −56 to −400 mV, the concentration of succinic acid in the fermented broth increased from
14 to 20.2 g/L；meanwhile, the concentration of lactic acid decreased from 44.9 to 35.2 g/L. The results of metabolic flux
analysis indicated the metabolic flux distribution at the glucose 6-phosphate and phosphoenolpyruvate nodes were affected
significantly. Compared with the value obtained at -56 mV, the flux of pentose phosphate pathway (HMP) pathway increased
by 2.74 fold and the flux from phosphoenolpyruvate to oxaloacetate increased by 78%. As a result, the flux of succinic acid
increased from 31.73 to 56.53 mmol/(L·g·h) and the flux of lactic acid decreased from 159.73 to 133.50 mmol/(L·g·h).
The results of this study demonstrated that glucose 6-phosphate and phosphoenolpyruvate were the key nodes that could
affect the production of succinic acid by C. crenatum fermentation under anaerobic conditions, which has contributed to the
co-production of lactic acid and succinic acid by manipulating wild-type C. crenatum to change the ratio of succinic acid/
lactic acid through metabolic engineering.

Key words: Corynebacterium crenatum, redox potential, metabolic flux analysis, anaerobic fermentation

中图分类号:

Q812

陈小举，操新民，姜绍通，李兴江. 氧化还原电位调控对钝齿棒杆菌代谢通量分布的影响[J]. 食品科学, doi: 10.7506/spkx1002-6630-201609031.

CHEN Xiaoju, CAO Xinmin, JIANG Shaotong, LI Xingjiang. Effect of Redox Potential Regulation on Metabolic Flux Distribution of Corynebacterium crenatum[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201609031.

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氧化还原电位调控对钝齿棒杆菌代谢通量分布的影响

Effect of Redox Potential Regulation on Metabolic Flux Distribution of Corynebacterium crenatum

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