食品科学 ›› 2019, Vol. 40 ›› Issue (1): 9-15.doi: 10.7506/spkx1002-6630-20170922-316

• 基础研究 • 上一篇    下一篇

百合鳞片褐变过程中膜脂过氧化研究

阚 娟,万 冰,解王晶,陈翠翠,刘 俊,金昌海*   

  1. 扬州大学食品科学与工程学院,江苏 扬州 225127
  • 出版日期:2019-01-15 发布日期:2019-01-22
  • 基金资助:
    国家自然科学基金青年科学基金项目(31101586);扬州大学科技创新培育基金项目(2016CXJ105)

Membrane Lipid Peroxidation during Browning of Lily Bulbs

KAN Juan, WAN Bing, XIE Wangjing, CHEN Cuicui, LIU Jun, JIN Changhai*   

  1. College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China
  • Online:2019-01-15 Published:2019-01-22

摘要: 以宜兴卷丹百合作为实验材料,研究百合鳞片采后低温贮藏过程中膜脂过氧化与褐变的关系。结果表明:在低温贮藏过程中,百合鳞片不同部位的褐变度、细胞膜透性、超氧阴离子自由基(O2-·)产生速率、H2O2和丙二醛含量均随着贮藏时间的延长呈上升趋势,且中层鳞片的上升速率始终低于外层和内层鳞片;内源过氧化物酶(peroxidase,POD)、超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)及脂氧合酶(lipoxygenase,LOX)活力呈先上升后下降趋势。中层鳞片中SOD和CAT活力始终高于外层和内层,而内源POD和LOX活力始终低于外层和内层鳞片;百合鳞片细胞活力在贮藏过程中逐渐降低,但中层鳞片的细胞活力始终高于外层和内层。百合鳞片褐变与膜脂过氧化密切相关,且不同部位百合鳞片在贮藏过程中褐变速率存在一定差异,表现为内层>外层>中层。本研究结果可为百合鳞片褐变机理的研究提供一定的实验依据。

关键词: 百合鳞片, 褐变, 膜脂过氧化

Abstract: The objective of this study was to relate membrane lipid peroxidation and browning in lily bulbs (Lilium lancifolium Thunb.) grown in Yixing, Jiangsu during postharvest cold storage. It was demonstrated that browning degree, cell membrane permeability, superoxide anion radicals production rate, hydrogen peroxide (H2O2) and malondialdehyde contents increased in all bulb parts with storage time. The rate of increase in these parameters was always higher in the middle layer than in the outer and inner layers of bulbs. The activities of exogenous peroxidase (POD), superoxide dismutase (SOD), catalase (CAT) and lipoxygenase (LOX) increased initially and decreased thereafter. The middle layer of bulbs showed higher SOD and CAT activities but lower POD and LOX activity than that of the two other layers. Cell viability in lily bulbs gradually decreased with storage time, and it was higher in the middle layer than in the two others. Browning was closely related to membrane lipid peroxidation in lily bulbs. The rate of browning was different among different bulb parts, in the decreasing order of inner > outer > middle layers. The results from this study can provide an experimental basis for understanding the mechanism of lily browning.

Key words: lily bulbs, browning, membrane lipid peroxide

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