食品科学 ›› 2019, Vol. 40 ›› Issue (21): 203-210.doi: 10.7506/spkx1002-6630-20181224-283

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采前喷施胺鲜酯对采后龙眼果实贮藏期间果皮膜脂代谢的影响

林毅雄,林河通,,陈艺晖,王慧,林艺芬,   

  1. (1.福建农林大学食品科学学院,福建 福州 350002;2.福建农林大学农产品产后技术研究所,福建 福州 350002;3.亚热带特色农产品采后生物学(福建农林大学)福建省高等学校重点实验室,福建 福州 350002)
  • 出版日期:2019-11-15 发布日期:2019-12-02
  • 基金资助:
    国家自然科学基金青年科学基金项目(31701653);国家自然科学基金面上项目(31671914;30972070;31171776); 福建省自然科学基金项目(2015J01608);福建省财政厅科技项目(KAA16010A;K81MLV01A); 福建农林大学科技创新专项(KF2015051;CXZX2017253;CXZX2017254)

Effect of Pre-Harvest Spraying of Diethyl Aminoethyl Hexanoate on Membrane Lipid Metabolism in Pericarp of Longan Fruits during Postharvest Storage

LIN Yixiong, LIN Hetong,, CHEN Yihui, WANG Hui, LIN Yifen,   

  1. (1. College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 2. Institute of Postharvest Technology of Agricultural Products, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 3. Key Laboratory of Postharvest Biology of Subtropical Special Agricultural Products (Fujian Agriculture and Forestry University), Fujian Province University, Fuzhou 350002, China)
  • Online:2019-11-15 Published:2019-12-02

摘要: 为研究采前喷施胺鲜酯(diethyl aminoethyl hexanoate,DA-6)对采后龙眼果实贮藏期间果皮膜脂代谢的影响,以‘福眼’龙眼为实验材料,在龙眼盛花期后70、90、110 d用10 mg/kg DA-6溶液各喷施龙眼果实1 次,以蒸馏水喷施为对照。龙眼果实在盛花期后120 d采收,采后龙眼果实经过挑选、清洗和晾干后用厚度为0.015 mm的聚乙烯薄膜袋密封包装(每组50 袋,每袋50 个),在(28±1)℃、相对湿度85%条件下贮藏。结果表明:采前喷施DA-6可延缓采后龙眼果实贮藏期间果皮细胞膜透性的增加,降低果皮脂氧合酶、脂酶和磷脂酶D等膜脂降解酶活力,延缓果皮膜磷脂(磷脂酰胆碱(phosphatidylcholine,PC)、磷脂酰肌醇(phosphatidylinositol,PI))和不饱和脂肪酸(油酸(C18:1)、亚油酸(C18:2)、亚麻酸(C18:3))的降解,保持较高的PC和PI含量及脂肪酸不饱和指数、脂肪酸不饱和度,保持较低的磷脂酸含量,维持正常细胞膜区室化功能和结构。因此,采前喷施DA-6增强采后龙眼果实耐贮性与采前喷施DA-6能有效降低采后龙眼果实贮藏期间果皮膜脂代谢有关。

关键词: 龙眼果实, 耐贮性, 胺鲜酯, 膜脂代谢, 膜脂降解酶, 膜脂组分

Abstract: In order to investigate the effect of pre-harvest spraying of diethyl aminoethyl hexanoate (DA-6) on membrane lipid metabolism in the pericarp of longan fruit during postharvest storage, ‘Fuyan’ longan (Dimocarpus longan Lour. cv. Fuyan) were sprayed with 10 mg/kg DA-6 or distilled water (as the control) once at 70, 90 and 110 days after full bloom. The fruit were harvested at 120 days, and they were selected, cleaned and dried prior to packaging in 0.015 mm thick polyethylene bags (50 fruits per bag, 50 bags per group) and subsequent storage at (28 ± 1) ℃ and 85% relative humidity. The results showed that compared with the control fruit, pre-harvest spraying of DA-6 could retard the increase in cell membrane permeability, reduce the activity of membrane lipid-degrading enzymes such as lipoxygenase (LOX), lipase and phospolipase D (PLD), maintain higher contents of phosphatidylcholine (PC) and phosphatidylinositol (PI), higher index of unsaturated fatty acids (IUFA) and relative degree of unsaturation of fatty acids (U/S), but lower content of phosphatidic acid (PA) in the pericarp of harvested longan fruit during storage. Therefore, pre-harvest spraying of DA-6 could delay the degradation of unsaturated fatty acids (oleic acid (C18:1), linoletic acid (C18:2) and linolenic acid (C18:3)) of cellular membrane lipids, which were beneficial to maintain the cellular structure. From these results, it could be concluded that improved longan fruit storability by pre-harvest spraying of DA-6 may be related to effectively alleviated membrane lipid metabolism in the pericarp of longan fruit during postharvest storage.

Key words: longan fruit, storability, diethyl aminoethyl hexanoate, membrane lipid metabolism, membrane lipid-degrading enzymes, membrane lipid composition

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