食品科学 ›› 2019, Vol. 40 ›› Issue (22): 110-117.doi: 10.7506/spkx1002-6630-20190520-225

• 生物工程 • 上一篇    下一篇

转谷氨酰胺酶的固定化及其在处理米糠废水中的应用

裴兴武,汪鸿,高子雯,袁泰增,高明,姚凯,潘明喆,于殿宇   

  1. (1.东北农业大学食品学院,黑龙江 哈尔滨 150030;2.湖北天星粮油股份有限公司,湖北 随州 441300)
  • 出版日期:2019-11-25 发布日期:2019-12-02
  • 基金资助:
    “十三五”国家重点研发计划重点专项(2018YFD0401101)

Immobilization of Transglutaminase and Its Application in the Treatment of Rice Bran Wastewater

PEI Xingwu, WANG Hong, GAO Ziwen, YUAN Taizeng, GAO Ming, YAO Kai, PAN Mingzhe, YU Dianyu   

  1. (1. College of Food Science, Northeast Agricultural University, Harbin 150030, China; 2. Hubei Tianxing Grain and Oil Co. Ltd., Suizhou 441300, China)
  • Online:2019-11-25 Published:2019-12-02

摘要: 制备醋酸纤维素-聚丙烯复合膜,并将转谷氨酰胺酶(transglutaminase,MTG)固定在膜上,制得了固定化MTG酶膜,测得其酶活为17.6 U/g。然后将酶膜固定在不锈钢网框上,并将其悬挂在烧杯中,用来处理米糠废水中的蛋白质。利用单因素试验,分别考察物料温度、物料pH值、转子转速以及酶膜面积对蛋白质聚合率的影响,并在单因素试验基础上,利用响应面试验优化,得到最佳聚合条件为物料温度43 ℃、pH 6.6、转子转速124 r/min、酶膜面积与底物含量比例为80∶1(cm2/g)、反应时间1.8 h,蛋白质聚合率为70%。固定化酶膜在使用5 次后,相对酶活力仍保持在74.3%以上,为连续聚合米糠废水中的蛋白质以及保留营养物质提供理论基础。

关键词: 转谷氨酰胺酶, 复合酶膜, 米糠废水, 蛋白质聚合率, 重复使用

Abstract: In this study, cellulose acetate-polypropylene composite membrane was prepared, and microbial transglutaminase (MTG) was immobilized onto the membrane, yielding an MTG-immobilized membrane, whose enzymatic activity was determined to be 17.6 U/g. Then the enzyme-loaded membrane was fixed on a stainless steel mesh frame and suspended in a beaker in order to use it to treat protein in rice bran wastewater. The effects of material temperature, material pH, rotor speed and enzyme-loaded membrane area on protein polymerization rate were investigated by one-factor-at-a-time method. Furthermore, using response surface optimization, the optimum polymerization conditions were obtained as follows: material temperature 43 ℃, pH 6.6, rotor speed 124 r/min, ratio between enzyme-loaded membrane area and substrate amount 80:1 (cm2/g), and reaction time 1.8 h. Under these conditions, protein polymerization rate was 70%. The enzyme-immobilized membrane remained above 74.3% of the original activity after its fifth repeated use. This study provides a theoretical basis for the continuous polymerization of proteins in rice bran wastewater and the retention of nutrients.

Key words: transglutaminase, enzyme-loaded composite membrane, rice bran wastewater, protein polymerization rate, repeated use

中图分类号: